catalog number :
MBS143065
products type :
Recombinant Protein
products full name :
Recombinant Human Involucrin
products short name :
Involucrin
products name syn :
Involucrin Human; Involucrin Human Recombinant; Involucrin; IVL; Involucrin
other names :
involucrin; Involucrin; involucrin; involucrin
other gene names :
IVL; IVL
uniprot entry name :
INVO_HUMAN
form :
Involucrin at 100 ug/ml in 50mM Tris-HCl, pH7.5, 10mM L-glutathione (reduced). Sterile Filtered clear solution.
storage stability :
Store vial at -20 degree C to -80 degree C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles.
tested application :
ELISA (EIA), Inhibition Assays, Western Blot (WB)
products categories :
RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins
products description :
Description: Involucrin Human Recombinant (aa 420-578) expressed in E Coli, shows a 41 kDa band on SDS-PAGE (Including GST tag).The Involucrin is purified by proprietary chromatographic techniques. Introduction: Involucrin is a soluble cytoplasmic protein precursor of the epidermal cornfied envelope that becomes cross-linked by transglutaminase during envelope assembly. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea which lacks a distinct cornfied layer. The protein is a useful marker of terminal differentiation. It is tightly linked to the onset of differentiation and first expressed in the immediate suprabasal layers of the epidermis. Involucrin is mapped to 1q21 chromosome, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin.Involucrin's structure is composed of a conserved region of about 75 amino acid residues followed by two very variable length segments that contain glutamine-rich tandem repeats. The glutamine residues in the tandem repeats are the substrate for the tranglutaminase in the cross-linking reaction. The size of the protein varies from 285 residues (in dog) to 835 residues (in orangutan).
ncbi acc num :
NP_005538.2
ncbi gb acc num :
NM_005547.2
ncbi mol weight :
68,479 Da
ncbi pathways :
Corticotropin-releasing Hormone Pathway (920957); Validated Transcriptional Targets Of AP1 Family Members Fra1 And Fra2 Pathway (169349)
ncbi summary :
Involucrin, a component of the keratinocyte crosslinked envelope, is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21, among calpactin I light chain, trichohyalin, profillaggrin, loricrin, and calcyclin. [provided by RefSeq, Jul 2008]
uniprot summary :
IVL: Part of the insoluble cornified cell envelope (CE) of stratified squamous epithelia. Belongs to the involucrin family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytoplasm; cornified envelope. Molecular Function: protein binding, bridging; structural molecule activity. Biological Process: keratinocyte differentiation; isopeptide cross-linking via N6-(L-isoglutamyl)-L-lysine; keratinization; peptide cross-linking; response to UV-B