protein

Recombinant Human Small Nuclear Ribonucleoprotein 70kDa

MBS142999

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Small Nuclear Ribonucleoprotein 70kDa

Small Nuclear Ribonucleoprotein

SNRNP70 Human; Small Nuclear Ribonucleoprotein 70kDa Human Recombinant; U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa; snRNP70; U1-70K; SNRNP70; RNPU1Z; RPU1; SNRP70; U1AP1; U1AP; U170K; U1RNP

U1 small nuclear ribonucleoprotein 70 kDa isoform 2; U1 small nuclear ribonucleoprotein 70 kDa; U1 small nuclear ribonucleoprotein 70 kDa; U1 snRNP 70 kDa; small nuclear ribonucleoprotein 70kDa (U1)

SNRNP70

SNRNP70; SNRNP70; RPU1; Snp1; U1AP; U170K; U1RNP; RNPU1Z; SNRP70; U1-70K; RNPU1Z; RPU1; SNRP70; U1AP1; U1 snRNP 70 kDa; U1-70K; snRNP70

RU17_HUMAN

E Coli

428

Greater than 90% as determined by SDS-PAGE.

U1-snRNP is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA and 0.02% SDS. Sterile Filtered clear solution.

0.3-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Western Blot (WB)

Western-Blot with monoclonal anti-hexa-His-tag antibody & MCTD sera (Mixed Connective Tissue Disease).

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Small Nuclear Ribonucleoprotein Polypeptide

Description: Recombinant Human U1-snRNP 68kDa cDNA codes for the 70kDa isoform of the human U1-snRNP 68 protein (lacking 66 internal amino acids outside the known epitope-containing areas) fused to a hexahistidine purification tag.The Calculated molecular weight is 44.8 kDa (U1-snRNP 68 displays aberrant electrophoretic mobility leading to an apparent discrepancy between calculated molecular weight and the 55-56 kDa molecular weight determined for this internally shortened molecule by SDS gel electrophoresis). Introduction: Small nuclear ribonucleoprotein complexes (abbreviated as U-snRNP) are essential for splicing of precursor mRNA molecules. U1-snRNP is the most abundant RNP particle in the nucleus and consists of one small uridylate-rich RNA (U1 RNA) complexed with several proteins: the three 68/70 kDa, A, C polypeptides are unique to the U1-snRNP particle, whereas 7 so-called Sm proteins (B/B', D1, D2, D3, E, F, G) form a core subparticle that is common to all U-snRNP complexes.Both the U1-specific proteins and the Sm core particle are targets of autoantibodies which classically have been called the RNP and RNP-Sm specificities, respectively. A clean diagnostic distinction of these specificities has been complicated by the biochemical difficulties of producing clean subparticle fractions from native sources. The use of single recombinant proteins as antigenic targets guarantees a much higher sensitivity and specificity and is the only way to determine RNP antibodies sensu stricto without the disturbing influence of Sm antigens; also with single U1 proteins antibodies will be detected which can be missed because of steric hindrance when using the RNP-Sm complex in an assay.Autoantibodies to U1-snRNP are present in 95% of patients with Mixed Connective Tissue Disease (MCTD) and 30% of patients with SLE. Antibodies against the 68/70 kDa protein are known to have a high clinical significance in MCTD patients. The 68/70 kDa nomenclature of this protein refers to the fact that different splice variants of the protein are found in human cells.

666637959

NP_001287998.1

NM_001301069.1

P08621

39,191 Da

Gene Expression Pathway (105937); Processing Of Capped Intron-Containing Pre-mRNA Pathway (160950); Spliceosome Pathway (125136); Spliceosome Pathway (124832); Spliceosome, U1-snRNP Pathway (413435); Spliceosome, U1-snRNP Pathway (890598); MRNA Splicing Pathway (105951); MRNA Splicing - Major Pathway (105952); MRNA Processing Pathway (198843)

snRNP 70: mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. Participates in pre-mRNA splicing and exonic splicing complexes. A major ribonucleoprotein antigen recognized by the sera from patients with autoimmune diseases, such as systemic lupus erythematosus. Four alternatively spliced human isoforms have been described. The truncated isoforms (2-4) cannot bind U1-snRNA. Protein type: RNA-binding; RNA splicing; Spliceosome. Chromosomal Location of Human Ortholog: 19q13.3. Cellular Component: nucleoplasm; spliceosome; nuclear speck; snRNP U1; nucleus. Molecular Function: protein binding; RNA binding; nucleotide binding. Biological Process: nuclear mRNA splicing, via spliceosome; positive regulation of nuclear mRNA splicing, via spliceosome; regulation of RNA splicing; RNA splicing; gene expression; mRNA processing

0.005 mg

140 USD

0.02 mg

205

1 mg

3395