Recombinant Rat Parvalbumin | MyBioSource MBS142953 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Rat Parvalbumin

catalog :

MBS142953

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142953

products type :

Recombinant Protein

products full name :

Recombinant Rat Parvalbumin

products short name :

Parvalbumin

products name syn :

PVALB Rat; Parvalbumin Rat Recombinant; Pvalb; Pva; PALB1; Parvalbumin

other names :

parvalbumin alpha; Parvalbumin alpha; parvalbumin alpha; Parvalbumin (calcium binding protein); parvalbumin

products gene name :

rPVALB

other gene names :

Pvalb; Pvalb; Pva; PALB1; Pva

uniprot entry name :

PRVA_RAT

host :

E Coli

sequence length :

110

purity :

Greater than 90% as determined by SDS-PAGE.

form :

The protein was lyophilized from a concentrated solution (1mg/ml) without any additives. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized Parvalbumin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Parvalbumin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

tested application :

Immunoblots, Immunohistochemistry (IHC), Radioimmunoassay (RIA), Intracellular Injection

app notes :

The Parvalbumin can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For fluid-phase adsorption in immunohistochemistry we suggest the following procedure: A- Dilute 1 ul of the monoclonal antibody 235 against parvalbumin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). B- Add 1 ug of the Parvalbumin to 1ml of the diluted antibody solution and mix well. C- Incubate for at least 6 hours in the cold. D- Apply to tissue-sections and incubate for 3 days. D- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized Parvalbumin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Parvalbumin

products description :

Description: Recombinant Rat Parvalbumin produced in E Coli.The Rat Parvalbumin is purified by proprietary chromatographic techniques. Introduction: Parvalbumin is calcium binding albumin protein, which binds two calcium ions. Parvalbumin has 3 EF hand motifs and is structurally related to calmodulin and troponin C. Pvalb is localized in fast-contracting muscles, where its levels are highest, in the brain and some endocrine tissues. Parvalbumin is found in GABAergic interneurons in the nervous system, primarily expressed by chandelier and basket cells in the cortex. Parvalbumin interneurons' connections are generally perisomatic (surrounding the cell body of neurons). In the hippocampus, PV+ interneurons are subdivided into basket, axo-axonic, bistratified, and oriens-lacunosum moleculare (O-LM) cells, each subtype targeting distinct domains of pyramidal cells. The majority of the Parvalbumin interneurons are fast-spiking, and are thought to generate gamma waves recorded in EEG. Pvalb -expressing interneurons represent roughly 25% of GABA cells in the primate DLPFC. Decreased Parvalbumin and GAD67 expression was found in PV+ GABAergic interneurons in schizophrenia. Parvalbumin is identified as an allergen causing seafood allergy. Pvalb may have evolved from an ancestral four domain calcium binding protein.

ncbi gi num :

11968064

ncbi acc num :

NP_071944.1

ncbi gb acc num :

NM_022499.2

uniprot acc num :

P02625

ncbi mol weight :

11,926 Da

ncbi summary :

binds two calcium ions; may have evolved from an ancestral four domain calcium binding protein [RGD, Feb 2006]

uniprot summary :

PVA: In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. Belongs to the parvalbumin family. Cellular Component: protein complex; cell soma; axon; cytoplasm; terminal button; nucleus. Molecular Function: protein homodimerization activity; protein heterodimerization activity; calcium ion binding. Biological Process: cytosolic calcium ion homeostasis

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

325

size3 :

0.1 mg

price3 :

2365

more info or order :

MyBioSource product webpage