Recombinant Human Ku P70/P80 | MyBioSource MBS142952 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Ku P70/P80

catalog :

MBS142952

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142952

products type :

Recombinant Protein

products full name :

Recombinant Human Ku P70/P80

products short name :

Ku P70/P80

products name syn :

Ku P70/P80 Human; Ku P70/P80 Human Recombinant; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; Lupus Ku autoantigen protein p70; Ku70; 70 kDa subunit of Ku antigen; Thyroid-lupus autoantigen; TLAA; CTC box-binding factor 75 kDa subunit; CTCBF; CTC75; DNA-repair protein XRCC6; G22P1; XRCC6; ML8

other names :

X-ray repair cross-complementing protein 6 isoform 1; X-ray repair cross-complementing protein 6; X-ray repair cross-complementing protein 6; 5'-dRP lyase Ku70; 5'-deoxyribose-5-phosphate lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase II, 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; DNA repair protein XRCC6; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; lupus Ku autoantigen protein p70; thyroid autoantigen 70kD (Ku antigen); thyroid autoantigen 70kDa (Ku antigen); thyroid-lupus autoantigen p70; X-ray repair complementing defective repair in Chinese hamster cells 6; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Lupus Ku autoantigen protein p70; Ku70; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 6

products gene name :

Ku P70/P80

other gene names :

XRCC6; XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1; G22P1; 5'-dRP lyase Ku70; CTC75; CTCBF; Ku70; TLAA

uniprot entry name :

XRCC6_HUMAN

host :

Sf9 Insect Cells

sequence length :

609

purity :

Greater than 90% as determined by SDS-PAGE.

form :

KuP70/P80is supplied in 16mM HEPES buffer pH-8, 160mM NaCl, and 20% glycerol. Sterile Filtered clear solution.

concentration :

0.2-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

tested application :

Western Blot (WB)

app notes :

Western-Blot with monoclonal anti-hexa-His-tag antibody & anti-Ku autoantibody-positive patient sera.

other info2 :

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

products description :

Description: KuP70/P80 Human Recombinant produced in SF9 insect cells is a glycosylated, polypeptide chain having a molecular mass of 70,638 Dalton for the p70 subunit and 83,528 Dalton for the p80 subunit. Ku (P70/P80) is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Introduction: Ku antigen consists of two polypeptides (70 kDa and 80 kDa; the p80 subunit is occasionally called p86). The Ku heterodimer provides DNA-binding and regulatory functions to DNA-dependent protein kinase which is a central enzyme for DNA recombination processes in eucaryotic cells. The Ku protein plays important roles in multiple nuclear processes including DNA repair, V(D)J recombination, telomere maintenance, and the regulation of specific gene transcription. Antibodies to Ku were originally reported in scleroderma-polymyositis overlap syndrome (up to 20% of patients) but have also been detectable in about 20 % of patients with primary pulmonary hypertension (as a complication of overlap), in 5-10 % of SLE and MCTD patients, and in up to 20 % of patients with primary Sj?gren's syndrome.In IIF using HEp-2 cells, Ku-positive sera yield a dense, fine granular pattern with nucleolar staining that is typical but not specific for anti-Ku. Detection of Ku antibodies by Western blot may bear false-negative results since the main reactive epitope on the p70/p80 heterodimer is conformation-dependent.

ncbi gi num :

573014815

ncbi acc num :

NP_001275905.1

ncbi gb acc num :

NM_001288976.1

uniprot acc num :

P12956

ncbi mol weight :

65,149 Da

ncbi pathways :

2-LTR Circle Formation Pathway (105993); BARD1 Signaling Events Pathway (137959); Coregulation Of Androgen Receptor Activity Pathway (138085); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); DNA Repair Pathway (105837); DNA-PK Complex Pathway (413430); DNA-PK Complex Pathway (890556); Disease Pathway (530764); Double-Strand Break Repair Pathway (105861); Early Phase Of HIV Life Cycle Pathway (105985)

ncbi summary :

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

uniprot summary :

Ku70: a mini-chromosome maintenance protein, essential for the initiation of eukaryotic genome replication. Allows DNA to undergo a single round of replication per cell cycle. Required for the entry in S phase and for cell division. Protein type: DNA repair, damage; EC 3.6.4.-; Nuclear receptor co-regulator; Helicase; EC 4.2.99.-; RNA-binding; DNA-binding. Chromosomal Location of Human Ortholog: 22q13.2. Cellular Component: nucleoplasm; transcription factor complex; nuclear telomere cap complex; membrane; nucleolus; cytosol; nucleus. Molecular Function: ATP-dependent DNA helicase activity; protein C-terminus binding; protein binding; DNA binding; double-stranded DNA binding; damaged DNA binding; double-stranded telomeric DNA binding; 5 -deoxyribose-5-phosphate lyase activity; ATP binding. Biological Process: positive regulation of neurogenesis; V(D)J recombination; viral reproduction; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; DNA repair; double-strand break repair via nonhomologous end joining; DNA duplex unwinding; DNA ligation; double-strand break repair; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; brain development; negative regulation of transcription, DNA-dependent; telomere maintenance

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3395

more info or order :

MyBioSource product webpage