protein

Recombinant Synaptosomal-associated protein 25kDa, C.elegans

MBS142951

0.01 mg

140 USD

Recombinant Protein

Recombinant Synaptosomal-associated protein 25kDa, C.elegans

Synaptosomal-associated protein

SNAP25 C.elegans; Synaptosomal-associated protein 25kDa C.elegans Recombinant; Super-Protein; SUP; RIC4; SEC9; SNAP; RIC-4; SNAP25; SNAP-25; Synaptosomal-associated protein 25; Synaptosomal-associated 25 kDa protein; FLJ23079; bA416N4.2; dJ1068F16.2

synaptosomal-associated protein 25 isoform a; Synaptosomal-associated protein 25; synaptosomal-associated protein 25; SNAP-25; SUP; super protein; synaptosomal-associated 25 kDa protein; synaptosomal-associated protein, 25 kDa; synaptosomal-associated protein 25; Super protein; SUP; Synaptosomal-associated 25 kDa protein

SNAP-25

Snap25; Snap25; SNAP-25B; SNAP-25a; Snap; SNAP-25; SUP

SNP25_RAT

E Coli

206

MSGDDDIPEG LEAINLKMNA TTDDSLESTR RMLALCEESK EAGIKTLVML DDQGEQLERCEGALDTINQD MKEAEDHLKG MEKCCGLCVL PWNKTDDFEK TEFAKAWKKD DDGGVISDQPRITVGDSSMG PQGGYITKIT NDAREDEMDE NVQQVSTMVG NLRNMAIDMS TEVSNQNRQL DRIHDKAQSN EVRVESANKR AKNLITK.

Greater than 95.0% as determined by SDS-PAGE.

The protein solution (1mg/ml) contains 20mM Tris-HCl pH7.5, 50mM NaCl, 5mM DTT, 1mM EDTA and 10% Glycerol. Sterile filtered colorless solution.

1mg/ml

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Synaptosomal Associated Protein

Description: Recombinant C.elegans SNAP-25 produced in E Coli is a single, non-glycosylated polypeptide chain containing 207 amino acids and having a molecular mass of 23kDa. SNAP-25 gene was amplified by PCR from C.elegans and cloned into an E Coli expression vector. SNAP-25 was purified by using conventional chromatography techniques. Introduction: Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, SNAP25 product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. The synaptosomal-associated protein (SNAP-25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP-25 is directly involved in the release of neurotransmitters.

396080314

NP_001257504.1

NM_001270575.1

23,336 Da

Acetylcholine Neurotransmitter Release Cycle Pathway (1121163); GABA Synthesis, Release, Reuptake And Degradation Pathway (1121164); Glutamate Neurotransmitter Release Cycle Pathway (1121161); Insulin Signaling Pathway (198452); Insulin Secretion Pathway (777547); Integration Of Energy Metabolism Pathway (1121025); Metabolism Pathway (1120894); Neuronal System Pathway (1121153); Neurotransmitter Release Cycle Pathway (1121158); Norepinephrine Neurotransmitter Release Cycle Pathway (1121159)

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein. It is essential for regulated exocytosis in neuronal cells. Alternative transcript variants encoding two different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2012]

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described. Protein type: Vesicle. Cellular Component: voltage-gated potassium channel complex; neuron projection; cell; intracellular organelle; terminal button; trans-Golgi network; cytosol; lipid raft; actin cytoskeleton; SNARE complex; growth cone; membrane; cell soma; axon; lamellipodium; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; cell junction; myelin sheath; endosome; vesicle; filopodium. Molecular Function: SNAP receptor activity; protein domain specific binding; voltage-gated potassium channel activity; protein binding; SNARE binding; myosin binding; syntaxin-1 binding; protein N-terminus binding; calcium-dependent protein binding. Biological Process: synaptic vesicle fusion to presynaptic membrane; growth hormone secretion; exocytosis; regulation of synaptogenesis; axonogenesis; long-term memory; neurotransmitter secretion; calcium ion-dependent exocytosis of neurotransmitter; positive regulation of hormone secretion; positive regulation of insulin secretion; endosome transport; sleep; synaptic vesicle priming

0.01 mg

140 USD

0.05 mg

205

1 mg

1790