protein

Recombinant Human Ribosomal Phosphoprotein P0

MBS142947

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Ribosomal Phosphoprotein P0

Ribosomal Phosphoprotein P0

RPLP0 Human; Ribosomal Phosphoprotein P0 Human Recombinant; 60S acidic ribosomal protein P0; Ribosomal Phosphoprotein P0; L10E; RPLP0; Ribosomal Protein Large P0; RPP0; P0; PRLP0; MGC88175; MGC111226

60S acidic ribosomal protein P0; 60S acidic ribosomal protein P0; 60S acidic ribosomal protein P0; 60S ribosomal protein L10E; acidic ribosomal phosphoprotein P0; ribosomal protein, large, P0; 60S ribosomal protein L10E

RPLP0

RPLP0; RPLP0; P0; LP0; L10E; RPP0; PRLP0

RLA0_HUMAN

Sf9 Insect Cells

317

Greater than 80% as determined by SDS-PAGE.

RPLP0 is supplied in 20mM HEPES buffer pH-7.5, 0.01mM EDTA & 0.02% SDS. Sterile Filtered clear solution.

0.3-0.7 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Western Blot (WB)

Western-Blot with monoclonal anti-hexa-His-tag antibody & SLE sera (Systemic Lupus Erythematodes Disease).

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Ribosomal Protein

Description: Ribosomal Phosphoprotein P0 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 35,096 Dalton. RPLP0 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. Introduction: The ribosomal phosphoproteins, also called P protein antigens, are associated with the large ribosomal subunit and therefore are antigenic targets with a cytoplasmic localization. Three P proteins have been described: P0 with a molecular weight of 35 kDa, P1 (19 kDa) and P2 (17 kDa). RPLP0 is a ribosomal protein that is a component of the 60S subunit. RPLP0 belongs to the L10P family of ribosomal proteins. RPLP0 is a neutral phosphoprotein having a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 & P2. The P0 protein interacts with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of RPLP0 scattered throughout the genome.Autoantibodies against ribosomal P proteins are present in 10 % of SLE patients. If anti-ribosomal P antibodies were to occur in the absence of other typical SLE associated autoantibodies, they may account for some patients with so-called ANA-negative lupus. It has been reported that lupus patients positive for anti-ribosomal P autoantibodies have a high frequency of CNS involvement, suggesting a marker use for these antibodies.

4506667

NP_000993.1

NM_001002.3

P05388

27,436 Da

Cap-dependent Translation Initiation Pathway (105967); Cytoplasmic Ribosomal Proteins Pathway (198853); Disease Pathway (530764); Eukaryotic Translation Elongation Pathway (105976); Eukaryotic Translation Initiation Pathway (105966); Eukaryotic Translation Termination Pathway (105978); Formation Of A Pool Of Free 40S Subunits Pathway (105968); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973); Gene Expression Pathway (105937); Influenza Infection Pathway (106067)

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein, which is the functional equivalent of the E. coli L10 ribosomal protein, belongs to the L10P family of ribosomal proteins. It is a neutral phosphoprotein with a C-terminal end that is nearly identical to the C-terminal ends of the acidic ribosomal phosphoproteins P1 and P2. The P0 protein can interact with P1 and P2 to form a pentameric complex consisting of P1 and P2 dimers, and a P0 monomer. The protein is located in the cytoplasm. Transcript variants derived from alternative splicing exist; they encode the same protein. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]

RPLP0: a ribosomal protein of the L10P family. Located in the cytoplasm and nucleus. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This protein is a component of the 60S subunit. Protein type: Ribosomal; Translation. Chromosomal Location of Human Ortholog: 12q24.2. Cellular Component: focal adhesion; membrane; cytoplasm; ribonucleoprotein complex; nucleus; cytosol. Molecular Function: protein binding; structural constituent of ribosome; RNA binding. Biological Process: SRP-dependent cotranslational protein targeting to membrane; translational elongation; cellular protein metabolic process; viral reproduction; translation; translational initiation; ribosome biogenesis and assembly; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination

0.005 mg

140 USD

0.02 mg

205

1 mg

3395