protein

Recombinant Human Centromere Protein-B

MBS142945

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Centromere Protein-B

Centromere Protein-B

CENPB Human; Centromere Protein B Human Recombinant; Major centromere autoantigen B; Centromere protein B; CENP-B; CENPB

major centromere autoantigen B; Major centromere autoantigen B; major centromere autoantigen B; CENP-B; centromere protein B, 80kDa; Centromere protein B; CENP-B

CENPB

CENPB; CENPB; CENP-B

CENPB_HUMAN

Sf9 Insect Cells

599

Greater than 95% as determined by SDS-PAGE.

CENPB is supplied at a concentration of (0.99mg/ml) in 20mM HEPES buffer pH-7.6, 250mM NaCl, and 20% glycerol. Sterile Filtered liquid formulation.

0.99mg/ml

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Western Blot (WB)

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Centromere Protein

Description: CENPB Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 80kDa. CENPB is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques. Introduction: Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.CENPB is the most important of the centromeric autoantigens: it has a molecular weight of approx. 66 kDa and plays an important role in the formation of the centromeric chromatin. CENPB antibodies are present in the sera of up to 80% of patients with CREST syndrome.

21735415

NP_001801.1

NM_001810.5

P07199

FOXM1 Transcription Factor Network Pathway (137935)

This gene product is a highly conserved protein that facilitates centromere formation. It is a DNA-binding protein that is derived from transposases of the pogo DNA transposon family. It contains a helix-loop-helix DNA binding motif at the N-terminus, and a dimerization domain at the C-terminus. The DNA binding domain recognizes and binds a 17-bp sequence (CENP-B box) in the centromeric alpha satellite DNA. This protein is proposed to play an important role in the assembly of specific centromere structures in interphase nuclei and on mitotic chromosomes. It is also considered a major centromere autoantigen recognized by sera from patients with anti-centromere antibodies. [provided by RefSeq, Jul 2008]

CENPB: Interacts with centromeric heterochromatin in chromosomes and binds to a specific subset of alphoid satellite DNA, called the CENP-B box. May organize arrays of centromere satellite DNA into a higher-order structure which then directs centromere formation and kinetochore assembly in mammalian chromosomes. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 20p13. Cellular Component: chromosome; nucleus; chromosome, pericentric region. Molecular Function: centromeric DNA binding; sequence-specific DNA binding; satellite DNA binding; chromatin binding. Biological Process: regulation of transcription, DNA-dependent

0.005 mg

140 USD

0.02 mg

205

1 mg

3775