Recombinant Human Centromere Protein-A | MyBioSource MBS142944 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Centromere Protein-A

catalog :

MBS142944

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142944

products type :

Recombinant Protein

products full name :

Recombinant Human Centromere Protein-A

products short name :

Centromere Protein-A

products name syn :

CENPA Human; Centromere Protein A Human Recombinant; Histone H3-like centromeric protein A; Centromere protein A; CENP-A; Centromere autoantigen A; CENPA

other names :

histone H3-like centromeric protein A isoform b; Histone H3-like centromeric protein A; histone H3-like centromeric protein A; centromere autoantigen A; centromere protein A, 17kDa; centromere-specific histone; centromere protein A; Centromere autoantigen A; Centromere protein A; CENP-A

products gene name :

CENPA

other gene names :

CENPA; CENPA; CenH3; CENP-A; CENP-A

uniprot entry name :

CENPA_HUMAN

host :

Sf9 Insect Cells

sequence length :

114

purity :

Greater than 90% as determined by SDS-PAGE.

form :

CENPA is supplied in 20mM HEPES buffer pH-8, 100mM sodium chloride, and 6M Urea. Sterile Filtered liquid formulation.

concentration :

1mg/ml

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

tested application :

Western Blot (WB)

app notes :

Western-Blot with monoclonal anti-hexa-His-tag antibody & Scleroderma patient sera.

other info2 :

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels including CDC international reference sera).

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Centromere Protein

products description :

Description: CENPA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 17,015 Dalton. CENPA is expressed with a -6x His tag and purified by proprietary chromatographic techniques. Introduction: Centromere proteins are a group of proteins which form and/or mediate the function of centromeres, the central structures of chromosomes to which spindle fibers/microtubuli attach and pull the chromosomes apart in cell division. Currently, 9 centromere proteins are known and designated CENPA to CENP-I. Most of the centromere proteins are targets of autoantibodies, the anti-centromere antibodies.CENPA is another important centromeric autoantigen in addition to CENPB: it has a molecular weight of approx. 20 kDa and is incorporated into centromeric chromatin due to its histone-like properties. CENPA antibodies are an important marker for correct diagnosis of Scleroderma / CREST syndrome in CENPB-autoantibody negative patients.

ncbi gi num :

109637779

ncbi acc num :

NP_001035891.1

ncbi gb acc num :

NM_001042426.1

uniprot acc num :

P49450

ncbi mol weight :

13,001 Da

ncbi pathways :

Aurora A Signaling Pathway (137925); Aurora B Signaling Pathway (138080); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Chromosome Maintenance Pathway (161048); Deposition Of New CENPA-containing Nucleosomes At The Centromere Pathway (161050); FOXM1 Transcription Factor Network Pathway (137935); M Phase Pathway (105812); Mitotic Anaphase Pathway (105818); Mitotic Metaphase And Anaphase Pathway (730317)

ncbi summary :

Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

CENPA: a basic nuclear protein of the histone H3 family. May act as a core histone necessary for the assembly of centromeres. Contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. Protein type: DNA-binding. Chromosomal Location of Human Ortholog: 2p23.3. Cellular Component: nucleoplasm; inner kinetochore of condensed chromosome; nucleosome; nucleus; cytosol; condensed nuclear chromosome, pericentric region; chromosome, pericentric region. Molecular Function: protein binding; DNA binding; protein heterodimerization activity; chromatin binding. Biological Process: nucleosome assembly; kinetochore assembly; DNA replication-independent nucleosome assembly at centromere; viral reproduction; establishment of mitotic spindle orientation; mitotic cell cycle

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3775

more info or order :

MyBioSource product webpage