protein

Recombinant Human Intrinsic Factor

MBS142930

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Intrinsic Factor

Intrinsic Factor

IF Human; Intrinsic Factor Human Recombinant; Gastric intrinsic factor; Intrinsic factor; INF; IF; GIF; IFMH; TCN3; Cobalamin/Vitamin B-12 binding transport protein; Intrinsic Factor

gastric intrinsic factor; Gastric intrinsic factor; gastric intrinsic factor; gastric intrinsic factor (vitamin B synthesis); Intrinsic factor; IF; INF

GIF; GIF; IF; INF; IFMH; TCN3; IFMH; IF; INF

IF_HUMAN

Sf9 Insect Cells

417

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The protein solution (0.7mg/ml) contains 20mM HEPES pH-8, 100mM NaCl, 20% Glycerol.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

Description: Intrinsic Factor Human Recombinant produced in baculovirus is a glycosylated, polypeptide chain containing having a molecular mass of 55,000 Dalton. The Intrinsic Factor is fused to a hexa-histidine at the C-terminus and purified by proprietary chromatographic techniques for removal of bound Vitamin B-12. Introduction: Intrinsic Factor is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12 in the terminal ileum. Vitamin B12 is essential for erythrocyte maturation and mutations in the Intrinsic Factor may lead to congenital pernicious anemia. Upon entry into the stomach, vitamin B12 binds to one of two B12 binding proteins present in the gastric fluid. In the less acidic environment of the small intestine, these proteins dissociate from the vitamin, allowing it to bind to intrinsic factor and enter the portal circulation through a receptor in the ileal mucosa specific for the B12-intrinsic factor complex.

32189398

NP_005133.2

NM_005142.2

P27352

42,698 Da

Cobalamin (Cbl, Vitamin B12) Transport And Metabolism Pathway (833804); Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009)

This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008]

GIF: Promotes absorption of the essential vitamin cobalamin (Cbl) in the ileum. After interaction with CUBN, the GIF-cobalamin complex is internalized via receptor-mediated endocytosis. Defects in GIF are the cause of hereditary intrinsic factor deficiency (IFD); also known as congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia. Belongs to the eukaryotic cobalamin transport proteins family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; lysosomal lumen; microvillus; apical plasma membrane; extracellular region; endosome. Molecular Function: cobalamin binding. Biological Process: vitamin metabolic process; cobalamin metabolic process; cobalamin transport; cobalt ion transport; water-soluble vitamin metabolic process. Disease: Intrinsic Factor Deficiency

0.002 mg

140 USD

0.01 mg

205

1 mg

4250