Recombinant Human Cytokeratin 20 | MyBioSource MBS142906 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Cytokeratin 20

catalog :

MBS142906

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142906

products type :

Recombinant Protein

products full name :

Recombinant Human Cytokeratin 20

products short name :

Cytokeratin 20

products name syn :

KRT20 Human; Cytokeratin 20 Human Recombinant; Keratin type I cytoskeletal 20; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT; KRT20; CD20; CK20; KRT21; MGC35423

other names :

keratin, type I cytoskeletal 20; Keratin, type I cytoskeletal 20; keratin, type I cytoskeletal 20; cytokeratin 20; cytokeratin-20; protein IT; keratin 20, type I; Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT

products gene name :

KRT20

other gene names :

KRT20; KRT20; K20; CD20; CK20; CK-20; KRT21; CK-20; K20

uniprot entry name :

K1C20_HUMAN

host :

E Coli

sequence length :

424

purity :

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein (1mg/1ml) was lyophilized after from a sterile solution containing 30mM Tris-HCL pH-8, 9.5M urea, 2mM DDT, 2mM EDTA and 10mM methylammonium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized KRT20 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution KRT20 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized KRT20 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Reconstitution: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCI, 2 mM dithiothreitol, 10 mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Cytokeratin

products description :

Description: Cytokeratin 20 Human Recombinant produced in E Coli is a single,non-glycosylated polypeptide chain having a molecular mass of 48,553 Dalton. The KRT20 is purified by proprietary chromatographic techniques. Introduction: KRT20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

ncbi gi num :

27894337

ncbi acc num :

NP_061883.1

ncbi gb acc num :

NM_019010.2

uniprot acc num :

P35900

ncbi mol weight :

48,487 Da

ncbi summary :

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

uniprot summary :

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: cytoplasm; intermediate filament. Molecular Function: protein binding; structural constituent of cytoskeleton. Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3490

more info or order :

MyBioSource product webpage