protein

Recombinant Human Cytokeratin 19

MBS142905

0.02 mg

205 USD

Recombinant Protein

Recombinant Human Cytokeratin 19

Cytokeratin 19

KRT19 Human; Cytokeratin 19 Human Recombinant; Keratin type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19; KRT19; CK19; K1CS; MGC15366; KRT19

keratin, type I cytoskeletal 19; Keratin, type I cytoskeletal 19; keratin, type I cytoskeletal 19; 40-kDa keratin intermediate filament; CK-19; cytokeratin 19; cytokeratin-19; keratin, type I, 40-kd; keratin 19, type I; Cytokeratin-19; CK-19; Keratin-19; K19

KRT19

KRT19; KRT19; K19; CK19; K1CS; CK-19; K19

K1C19_HUMAN

E Coli

400

The protein (1mg/ml) was lyophilized after from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM DTT, 2mM EDTA and 10mM methylammonium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized KRT19 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution KRT19 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized KRT19 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Reconstitution: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Cytokeratin

Description: Cytokeratin 19 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain having a molecular mass of 44,098 Dalton. The KRT19 is purified by proprietary chromatographic techniques. Introduction: CTK-19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21.

24234699

NP_002267.2

NM_002276.4

P08727

44,106 Da

Signaling Mediated By P38-alpha And P38-beta Pathway 138012

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]

0.02 mg

205 USD