Recombinant Human Cytokeratin 18 | MyBioSource MBS142904 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Cytokeratin 18

catalog :

MBS142904

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142904

products type :

Recombinant Protein

products full name :

Recombinant Human Cytokeratin 18

products short name :

Cytokeratin 18

products name syn :

KRT18 Human; Cytokeratin 18 Human Recombinant; Keratin type I cytoskeletal 18; Cytokeratin-18; CK-18; Keratin-18; K18; KRT18; CYK18; Cell proliferation-inducing gene 46 protein

other names :

keratin, type I cytoskeletal 18; Keratin, type I cytoskeletal 18; keratin, type I cytoskeletal 18; CK-18; cell proliferation-inducing gene 46 protein; cell proliferation-inducing protein 46; cytokeratin 18; cytokeratin-18; keratin 18, type I; Cell proliferation-inducing gene 46 protein; Cytokeratin-18; CK-18; Keratin-18; K18

products gene name :

KRT18

other gene names :

KRT18; KRT18; K18; CYK18; CYK18; CK-18; K18

uniprot entry name :

K1C18_HUMAN

host :

E Coli

sequence length :

430

purity :

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The protein (1mg/ml) was lyophilized from a sterile solution containing 30mM Tris-HCl pH-8, 9.5M urea, 2mM EDTA, 2mM DTT and 10mM methylammonium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Lyophilized CK-18 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution CK-18 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

other info2 :

Solubility: It is recommended to reconstitute the lyophilized CK-18 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Reconstitution: Performed by mixing equimolar amounts of cytokeratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCI, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Cytokeratin

products description :

Description: Cytokeratin 18 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain having a calculated molecular mass of 48,201 Dalton, showing a 45kDa band on SDS-page, pI-5.7.The KRT18 is purified by proprietary chromatographic techniques. Introduction: KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

ncbi gi num :

4557888

ncbi acc num :

NP_000215.1

ncbi gb acc num :

NM_000224.2

uniprot acc num :

P05783

ncbi mol weight :

48,058 Da

ncbi pathways :

Caspase Cascade In Apoptosis Pathway (137974); EGFR1 Signaling Pathway (198782); Pathogenic Escherichia Coli Infection Pathway (83103); Pathogenic Escherichia Coli Infection Pathway (672459); Pathogenic Escherichia Coli Infection Pathway (516)

ncbi summary :

KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis. Protein type: Nucleolus; Cytoskeletal. Chromosomal Location of Human Ortholog: 12q13. Cellular Component: perinuclear region of cytoplasm; keratin filament; cytoplasm; nucleolus; intermediate filament; microtubule organizing center. Molecular Function: protein binding; structural molecule activity. Biological Process: anatomical structure morphogenesis; tumor necrosis factor-mediated signaling pathway; viral reproduction; intermediate filament cytoskeleton organization and biogenesis; cell cycle; negative regulation of apoptosis; Golgi to plasma membrane CFTR protein transport. Disease: Cirrhosis, Familial

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3490

more info or order :

MyBioSource product webpage