This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
MyBioSource
product type :
protein
product name :
Bovine Calpain-2 Catalytic Subunit
catalog :
MBS142885
quantity :
0.01 mg
price :
610 USD
product information
catalog number :
MBS142885
products type :
Native Protein
products full name :
Bovine Calpain-2 Catalytic Subunit
products short name :
Calpain-2 Catalytic Subunit
products name syn :
CAPN2; Bovine Calpain-2 Catalytic Subunit; Calpain-2 catalytic subunit; EC 3.4.22.53; Calpain-2 large subunit; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; M-calpain; Millimolar-calpain; CAPN2
other names :
calpain-2 catalytic subunit; Calpain-2 catalytic subunit; calpain-2 catalytic subunit; CANP 2; M-calpain; calcium-activated neutral proteinase 2; calpain M-type; calpain, large polypeptide L2; calpain-2 large subunit; millimolar-calpain; calpain 2, (m/II) large subunit<; Calcium-activated neutral proteinase 2; CANP 2; Calpain M-type; Calpain-2 large subunit; Millimolar-calpain; M-calpain
products gene name :
bCAPN2
other gene names :
CAPN2; CAPN2; CANP 2; M-calpain
uniprot entry name :
CAN2_BOVIN
host :
Bovine Myocardium
sequence length :
700
purity :
Greater than 90% as determined by SDS-PAGE.
form :
50mM Imidazole-HCl, 0.2mM EDTA, 1mM DTT and 50% glycerol, pH 7.4. Sterile Filtered colorless solution.
tested application :
Immunoblots, Immunohistochemistry (IHC), Radioimmunoassay (RIA), Intracellular Injection
app notes :
This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedure:A- Dilute 1 ul of the antiserum against m-calpain in 1ml of the usual buffer for immunohistochemistry (final dilution 1:1,000).B- Add 1 ug of protein to 1ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented.
products categories :
RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Calpain
products description :
Description: CAPN2 consists of an 80 kDa large subunit and a 30 kDa small subunit. CAPN2 was purified by sequential chromatography through DEAE-Sepharose, A 1.5m Bio-Gel, and Phenyl-Sepharose CL-4B columns. Introduction: Calpain's activity is attributed to two main isoforms: u-calpain and m-calpain, which are ubiquitously expressed proteases implicated in cellular migration, cell cycle progression, degenerative processes and cell death. These heterodimeric enzymes are composed of distinct catalytic subunits, encoded by Capn1 (u-calpain) or Capn2 (m-calpain), and a common regulatory subunit encoded by Capn4. Calpain2 (M-calpain) is believed to be membrane bound and functions at the trailing edge of the migrating cell to cleave the integrins in response to growth factor receptor signals. PKA functions to down regulate or inhibit calpain2.
ncbi gi num :
198282013
ncbi acc num :
NP_001096556.1
ncbi gb acc num :
NM_001103086.1
uniprot acc num :
Q27971
ncbi mol weight :
79,935 Da
ncbi pathways :
Alzheimer's Disease Pathway 84336!!Alzheimer's Disease Pathway 509!!Apoptosis Pathway 84299!!Apoptosis Pathway 470!!Focal Adhesion Pathway 84306!!Focal Adhesion Pathway 478!!Integrin-mediated Cell Adhesion Pathway 198555!!Protein Processing In Endoplasmic Reticulum Pathway 167316!!Protein Processing In Endoplasmic Reticulum Pathway 167190
size :
0.01 mg
price :
610 USD
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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