protein

Recombinant Human Tripartite Motif Containing 21 (RO52)

MBS142883

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Tripartite Motif Containing 21 (RO52)

Tripartite Motif Containing 21

TRIM21 Human; Tripartite Motif Containing 21 (RO52) Human Recombinant; 52 kDa Ro protein; Sjoegren syndrome type A antigen; SS-A; Ro(SS-A); 52 kDa ribonucleoprotein autoantigen Ro/SS-A; Tripartite motif-containing protein 21; RING finger protein 81; TRIM21; RNF81; RO52; SSA1; SSA; RO-52

E3 ubiquitin-protein ligase TRIM21; E3 ubiquitin-protein ligase TRIM21; E3 ubiquitin-protein ligase TRIM21; 52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING finger protein 81; Sicca syndrome antigen A; Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro); ro(SS-A); sjoegren syndrome type A antigen; tripartite motif-containing 21; tripartite motif-containing protein 21; tripartite motif containing 21; 52 kDa Ro protein; 52 kDa ribonucleoprotein autoantigen Ro/SS-A; RING finger protein 81; Ro(SS-A); Sjoegren syndrome type A antigen; SS-A; Tripartite motif-containing protein 21

TRIM21

TRIM21; TRIM21; SSA; RO52; SSA1; RNF81; Ro/SSA; RNF81; RO52; SSA1; SS-A

RO52_HUMAN

Sf9 Insect Cells

475

Greater than 90% as determined by SDS-PAGE.

TRIM21 is supplied in 20mM HEPES pH-7.5, 0.01mM EDTA and 0.02% SDS. Sterile Filtered clear solution.

Coating Concentration: 0.3-0.65 ug/ml (depending on the type of ELISA plate and coating buffer). Suitable for labeling of functional groups.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Tripartite Motif

Description: TRIM21 Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a calculated molecular mass of 54,922 Dalton. TRIM21 is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques. Introduction: TRIM21 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The 52 kDa Ro protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. Ro/SSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Ribonucleoprotein particle is composed of a single polypeptide and one of four small RNA molecules. The RoSSA is present in all mammalian cells studied but has no known function. At least 2 isoforms are present in nucleated and red blood cells, and tissue specific differences in Ro/SSA proteins were identified.

15208660

NP_003132.2

NM_003141.3

P19474

45,057 Da

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Cytokine Signaling In Immune System Pathway (366171); Cytosolic Sensors Of Pathogen-associated DNA Pathway (576255); Immune System Pathway (106386); Innate Immune System Pathway (106387); Interferon Signaling Pathway (187103); Interferon Gamma Signaling Pathway (187106); Regulation Of Innate Immune Responses To Cytosolic DNA Pathway (833823)

This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

TRIM21: E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2. Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination. Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes. A TRIM21-containing SCF(SKP2)- like complex is shown to mediate ubiquitination of CDKN1B ( Thr- 187 phosphorylated-form), thereby promoting its degradation by the proteasome. Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling. Negatively regulates IFN-beta production post-pathogen recognition by polyubiquitin- mediated degradation of IRF3. Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway. Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages. Plays a role in the regulation of the cell cycle progression. Enhances the decapping activity of DCP2. Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules. At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified. The common feature of these proteins is their ability to bind HY RNAs.2. Belongs to the TRIM/RBCC family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ligase; Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: nucleoplasm; cytoplasm; SCF ubiquitin ligase complex; cytosol; ribonucleoprotein complex; nucleus. Molecular Function: identical protein binding; protein binding; DNA binding; zinc ion binding; RNA binding; ubiquitin-protein ligase activity; ligase activity. Biological Process: protein monoubiquitination; regulation of interferon type I production; protein autoubiquitination; protein polyubiquitination; protein destabilization; cytokine and chemokine mediated signaling pathway; protein ubiquitination; positive regulation of cell cycle; cell cycle; negative regulation of viral transcription; positive regulation of virion penetration into host cell; inhibition of NF-kappaB transcription factor; positive regulation of interferon type I production; innate immune response; positive regulation of transcription factor activity

0.005 mg

140 USD

0.02 mg

205

1 mg

3490