protein

Recombinant Human Vimentin

MBS142865

0.02 mg

140 USD

Recombinant Protein

Recombinant Human Vimentin

Vimentin

Vimentin Human; Vimentin Human Recombinant; Vimentin; Vim; FLJ36605; Vimentin

vimentin; Vimentin; vimentin; epididymis luminal protein 113; vimentin

VIM; VIM; HEL113; CTRCT30

VIME_HUMAN

E Coli

466

mstrsvssss yrrmfggpgt asrpsssrsy vttstrtysl gsalrpstsr slyasspggv yatrssavrl rssvpgvrll qdsvdfslad aintefkntr tnekvelqel ndrfanyidk vrfleqqnki llaeleqlkg qgksrlgdly eeemrelrrq vdqltndkar veverdnlae dimrlreklq eemlqreeae ntlqsfrqdv dnaslarldl erkveslqee iaflkklhee eiqelqaqiq eqhvqidvdv skpdltaalr dvrqqyesva aknlqeaeew ykskfadlse aanrnndalr qakqesteyr rqvqsltcev dalkgtnesl erqmremeen faveaanyqd tigrlqdeiq nmkeemarhl reyqdllnvk maldieiaty rkllegeesr islplpnfss lnlretnlds lplvdthskr tlliktvetr dgqvinetsq hhddle.

Greater than 95.0% as determined by SDS-gelelctrophoresis.

The protein (1mg/ml) was lyophilized with 30mM Tris/HCl, pH 8, 9.5M urea, 2mM EDTA, 2mM DTT 10mM methylammonium chloride. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized Vimentin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Vimentin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the Vimentin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Reconstitution: After vimentin is dissolved in 9.5M urea buffer (see above), protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM NaCl, 2mM dithiothreitol, 10mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).- Hatzfeld M. and Franke W.W. (1985). J. Cell Biol. 101, 1826-1841- Hatzfeld M. et al. (1987). J. Mol. Biol. 197, 237-255.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Vimentin

Description: Vimentin Human Recombinant is produced in E Coli, having a molecular weight of 53,685 Dalton (calculated from sequence), 57,000 Dalton (determined by SDS gelelctrophoresis). Introduction: Vimentin expression in human malignant glioma cells depends on cellular density, algorithms of drug delivery and chemo/radio treatment. Vimentin and detyrosinated microtubules provide structural support for the extensive microtentacles observed in detached tumor cells and a mechanism to promote successful metastatic spread. Primary colorectal carcinomas display aberrant expression of vimentin, and have activated Notch and TGFbeta signaling pathways. Vimentin is a strong arterial substrate for transglutaminases. Transglutaminase-mediated vimentin dimerization results in a novel unifying pathway by which vasodilatory and remodeling responses may be regulated. Ablation of vimentin expression inhibits migration and invasion of colon and breast cancer cell lines. Vimentin is the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms.

62414289

NP_003371.2

NM_003380.3

P08670

53,652 Da

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Apoptotic Cleavage Of Cellular Proteins Pathway (105678); Apoptotic Execution Phase Pathway (105677); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (105679); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852705)

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

Vimentin: an intermediate filament protein. Intermediate filament proteins are expressed in a tissue-specific manner. Desmin is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue. Protein type: Motility/polarity/chemotaxis; Cytoskeletal. Chromosomal Location of Human Ortholog: 10p13. Cellular Component: intermediate filament cytoskeleton; neuron projection; focal adhesion; cytoskeleton; cytoplasm; leading edge; plasma membrane; intermediate filament; peroxisome; cytosol. Molecular Function: protein C-terminus binding; identical protein binding; protein binding; structural constituent of cytoskeleton; double-stranded RNA binding; glycoprotein binding; structural constituent of eye lens. Biological Process: Bergmann glial cell differentiation; viral reproduction; apoptosis; intermediate filament organization; cell motility; astrocyte development; muscle filament sliding; cell structure disassembly during apoptosis. Disease: Cataract 30

0.02 mg

140 USD

0.1 mg

205

1 mg

1095