Recombinant Human Fragile Histidine Triad, GST Tag | MyBioSource MBS142853 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Fragile Histidine Triad, GST Tag

catalog :

MBS142853

quantity :

0.002 mg

price :

265 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142853

products type :

Recombinant Protein

products full name :

Recombinant Human Fragile Histidine Triad, GST Tag

products short name :

Fragile Histidine Triad

products name syn :

FHIT Human, GST; Fragile Histidine Triad Human Recombinant, GST Tag; Bis(5'-adenosyl)-triphosphatase; EC 3.6.1.29; Diadenosine 5'; 5'''-P1; P3-triphosphate hydrolase; Dinucleosidetriphosphatase; AP3A hydrolase; AP3Aase; Fragile histidine triad protein; FHIT; FRA3B; FHIT, GST

other names :

bis(5'-adenosyl)-triphosphatase; Bis(5'-adenosyl)-triphosphatase; bis(5'-adenosyl)-triphosphatase; bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; diadenosine 5',5'''-P1,P3-triphosphate hydrolase; dinucleosidetriphosphatase; tumor suppressor protein; fragile histidine triad; AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

products gene name :

FHIT

other gene names :

FHIT; FHIT; FRA3B; AP3Aase; AP3Aase

uniprot entry name :

FHIT_HUMAN

host :

E Coli

sequence length :

147

form :

FHIT in 50mM Tris-Acetate, pH7.5, 1mM EDTA and 20% Glycerol. Sterile Filtered clear solution.

storage stability :

Store vial at -20 degree C to -80 degree C. When stored at the recommended temperature, this protein is stable for 12 months.Please prevent freeze-thaw cycles.

tested application :

ELISA (EIA), Inhibition Assays, Western Blot (WB)

products categories :

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins; Fragile Histidine Triad

products description :

Description: FHIT Human Recombinant full length protein expressed in E Coli, shows a 43 kDa band on SDS-PAGE. The FHIT is fused to GST-Tag and purified by proprietary chromatographic techniques. Introduction: FHIT is a member of the histidine triad gene family. FHIT gene encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. FHIT gene includes the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and abnormal transcripts of this gene. The FHIT protein is a tumor suppressor with reduced or no expression in numerous types of cancer. FHIT may also act as a tumor suppressor in normal cells. Alterations and deletions of the FHIT (Fragile Histidine Triad) gene are strongly linked to the genesis and establishment of human tumors of the lung, cervix, breast, colon, stomach, and pancreas. The expression of FHIT is more often lost in cancers of individuals with familial mutations causing deficiency in DNA repair genes such as BRCA1, BRCA2 and MSH2. In vitro FHIT functions as a hydrolase that cleaves diadenosine triphosphate (Ap3A) to ADP and AMP. The FHIT -Ap3A enzyme-substrate complex seems to be the tumor suppressor signal. The restoration of FHIT expression in FHIT -deficient cancer cells leads to apoptosis, involving the intrinsic caspase pathway, in cancer-derived cells and in tumor xenografts.

ncbi gi num :

261278358

ncbi acc num :

NP_001159715.1

ncbi gb acc num :

NM_001166243.1

uniprot acc num :

P49789

ncbi mol weight :

16,858 Da

ncbi pathways :

Non-small Cell Lung Cancer Pathway (83119); Non-small Cell Lung Cancer Pathway (531); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (307); Small Cell Lung Cancer Pathway (83118); Small Cell Lung Cancer Pathway (530)

ncbi summary :

This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009]

uniprot summary :

FHIT: a member of the histidine triad gene family. A diadenosine 5 ,5 -P1,P3-triphosphate hydrolase involved in purine metabolism. Its gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. A possible tumor suppressor in specific tissues. Phospho-FHIT observed in liver and kidney, but not in brain and lung. Phospho-FHIT undetected in all tested human tumor cell lines. Aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Protein type: EC 3.6.1.29; Hydrolase; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; DNA replication; Tumor suppressor. Chromosomal Location of Human Ortholog: 3p14.2. Cellular Component: mitochondrion; cytoplasm; nucleus; cytosol. Molecular Function: identical protein binding; protein binding; hydrolase activity; ubiquitin protein ligase binding; nucleotide binding; bis(5 -adenosyl)-triphosphatase activity; catalytic activity. Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; nucleotide metabolic process; DNA replication; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; purine nucleotide metabolic process

size1 :

0.002 mg

price1 :

265 USD

size2 :

0.005 mg

price2 :

365

size3 :

0.01 mg

price3 :

610

more info or order :

MyBioSource product webpage