protein

Recombinant Human Endostatin

MBS142835

0.02 mg

140 USD

Recombinant Protein

Recombinant Human Endostatin

Endostatin

Endostatin Human; Endostatin Human Recombinant; Endostatin

collagen alpha-1(XVIII) chain isoform 1 preproprotein; Collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; collagen alpha-1(XVIII) chain; antiangiogenic agent; endostatin; multi-functional protein MFP; collagen, type XVIII, alpha 1

COL18A1; COL18A1; KS; KNO; KNO1

COIA1_HUMAN

Pichia Pastoris

1516

Greater than 98.0% as determined by (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The recombinant Human Endostatin is formulated with PBS. Sterile filtered liquid formulation.

20ug/4ul

Endostatin although stable at 4 degree C for 30 days, should be stored desiccated below -20 degree C for periods greater than 30 days. Please avoid Freeze/Thaw cycles.

Biological Activity: The activity calculated by ECE migration inhibition was found to be 50,000IU/mg.

RECOMBINANT & NATURAL PROTEINS; Recombinant Proteins

Description: Endostatin Human Recombinant produced in Pichia Pastoris is a single, non-glycosylated, polypeptide having a total molecular mass of 20,000 Dalton C-terminal fragment of collagen XVIII that has been shown to act as a potent inhibitor of angiogenesis and tumor growth in vitro and in vivo. Induces tyrosine phosphorylation of Shc (SH2 domain adapter protein) leading to specific inhibition in endothelial cell prolifiration.The Endostatin is fused to His tag at N-terminus and purified by proprietary chromatographic techniques. Introduction: Once a cluster of cancer cells reaches a diameter of about 12 mm, it must develop a blood supply in order to grow larger. Given that diffusion is no longer adequate to supply the cancerous cells with oxygen and nutrients and to remove wastes. Therefore, these cells secrete substances that promote the formation of new blood vessels (angiogenesis), which are vital for the growth and persistence of solid tumors and their metastases.The production of several angiogenic factors (FGF-a, FGF-b, VEGF and VPF) is upregulated by tumors in order to stimulate angiogenesis. However, numerous malignant tumors also generate inhibitors of angiogenesis which include angiostatin and thrombospondin. The angiogenic phenotype is basically the end result of a net balance between the positive and negative regulators of neovascularization mentioned above. Endostatin's role is to inhibit endothelial proliferation, therefore it is an effective angiogenesis inhibitor.

110611235

NP_085059.2

NM_030582.3

135,761 Da

Activation Of Matrix Metalloproteinases Pathway (576264); Assembly Of Collagen Fibrils And Other Multimeric Structures Pathway (730306); Collagen Biosynthesis And Modifying Enzymes Pathway (645289); Collagen Degradation Pathway (730309); Collagen Formation Pathway (645288); Degradation Of The Extracellular Matrix Pathway (576263); Direct P53 Effectors Pathway (137939); Extracellular Matrix Organization Pathway (576262); FOXA1 Transcription Factor Network Pathway (137979); Integrin Cell Surface Interactions Pathway (106110)

This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]

COL18A1: COLA18A probably plays a major role in determining the retinal structure as well as in the closure of the neural tube. Defects in COL18A1 are a cause of Knobloch syndrome type 1 (KNO1). An autosomal recessive disorder defined by the occurrence of high myopia, vitreoretinal degeneration with retinal detachment, macular abnormalities and occipital encephalocele. Belongs to the multiplexin collagen family. 3 isoforms of the human protein are produced by alternative promoter. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 21q22.3. Cellular Component: extracellular matrix; extracellular space; collagen; endoplasmic reticulum lumen; extracellular region; basement membrane. Molecular Function: identical protein binding; protein binding; metal ion binding; structural molecule activity. Biological Process: response to drug; extracellular matrix disassembly; negative regulation of cell proliferation; collagen catabolic process; extracellular matrix organization and biogenesis; organ morphogenesis; visual perception; positive regulation of cell proliferation; endothelial cell morphogenesis; angiogenesis; cell adhesion; response to hydrostatic pressure; positive regulation of cell migration. Disease: Knobloch Syndrome 1

0.02 mg

140 USD

0.1 mg

205

1 mg

1030