Recombinant Human cyclin-dependent kinase 4 | MyBioSource MBS142819 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human cyclin-dependent kinase 4

catalog :

MBS142819

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142819

products type :

Recombinant Protein

products full name :

Recombinant Human cyclin-dependent kinase 4

products short name :

cyclin-dependent kinase 4

products name syn :

CDK-4 Human; Cyclin-Dependent Kinase 4 Human Recombinant; Cell division protein kinase 4; CDK4; EC 2.7.11.22; Cyclin-dependent kinase 4; PSK-J3; CMM3; CDK-4; MGC14458

other names :

cyclin-dependent kinase 4; Cyclin-dependent kinase 4; cyclin-dependent kinase 4; cell division protein kinase 4; cyclin-dependent kinase 4; Cell division protein kinase 4; PSK-J3

products gene name :

CDK4

other gene names :

CDK4; CDK4; CMM3; PSK-J3

uniprot entry name :

CDK4_HUMAN

host :

E Coli

sequence length :

303

purity :

Greater than 95% as determined by SDS-PAGE. Single band on Western Blot.

form :

CDK4 is supplied in 10mM Bis-Tris Propane pH9.0 and 50% Glycerol. Sterile Filtered clear solution.

storage stability :

Store at 4 degree C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

products categories :

PROTEIN KINASES; Enzymes; Cyclin-Dependent Kinase

products description :

Description: Cyclin-Dependent Kinase 4 Human Recombinant is expressed in E Coli as a full-length protein having a molecular weight of 38 kDa fused to an amino terminal hexahistidine tag. The CDK4 is purified by proprietary chromatographic techniques. Introduction: Cyclin-dependent kinase 4 combines with the protein cyclin D to form a protein complex that promotes the passage of cells through the G1 checkpoint in the cell growth cycle. This cellular activity is regulated by the protein p16 produced by p16INK4a. Normally, p16 controls cell growth by inhibiting the activity of the CDK4cyclin D complex and stopping cells at the G1 checkpoint.

ncbi gi num :

4502735

ncbi acc num :

NP_000066.1

ncbi gb acc num :

NM_000075.3

uniprot acc num :

P11802

ncbi mol weight :

20,725 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); B Cell Receptor Signaling Pathway (198909); Bladder Cancer Pathway (83115); Bladder Cancer Pathway (527); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463)

ncbi summary :

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is highly similar to the gene products of S. cerevisiae cdc28 and S. pombe cdc2. It is a catalytic subunit of the protein kinase complex that is important for cell cycle G1 phase progression. The activity of this kinase is restricted to the G1-S phase, which is controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). This kinase was shown to be responsible for the phosphorylation of retinoblastoma gene product (Rb). Mutations in this gene as well as in its related proteins including D-type cyclins, p16(INK4a) and Rb were all found to be associated with tumorigenesis of a variety of cancers. Multiple polyadenylation sites of this gene have been reported. [provided by RefSeq, Jul 2008]

uniprot summary :

CDK4: a protein kinase of the CDK family that is important for cell cycle G1 phase progression. Its activity is restricted to the G1-S phase. Controlled by the regulatory subunits D-type cyclins and CDK inhibitor p16(INK4a). Phosphorylates the retinoblastoma gene product (Rb). Point mutations found in somatic and familial melanoma. Amplified in sarcomas, glioma and lymphoma. Amplified, methylated or deleted in head and neck squamous cell carcinoma. Overexpression drives epithelial tumors in mice. Disruption makes mice resistant to cancer. Inhibitor: PD332991. Protein type: EC 2.7.11.22; Cell cycle regulation; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; CMGC group; CDK family; CDK4 subfamily; CDK/CDK4 subfamily. Chromosomal Location of Human Ortholog: 12q14. Cellular Component: nucleoplasm; transcription factor complex; nuclear membrane; tight junction; perinuclear region of cytoplasm; nucleolus; cyclin-dependent protein kinase holoenzyme complex; nucleus; cytosol; chromatin. Molecular Function: protein binding; cyclin binding; cyclin-dependent protein kinase activity; protein complex binding; cyclin-dependent protein kinase regulator activity; ATP binding. Biological Process: response to drug; lens development in camera-type eye; circadian rhythm; establishment and/or maintenance of chromatin architecture; organ regeneration; positive regulation of cell size; positive regulation of translation; positive regulation of apoptosis; response to toxin; response to testosterone stimulus; signal transduction; protein amino acid phosphorylation; positive regulation of fibroblast proliferation; response to hyperoxia; regulation of gene expression; cell division; response to lead ion; positive regulation of cell proliferation; mitotic cell cycle; regulation of protein kinase activity; G1/S transition of mitotic cell cycle. Disease: Melanoma, Cutaneous Malignant, Susceptibility To, 3

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4960

more info or order :

MyBioSource product webpage