protein

Recombinant Human Jun Proto-Oncogene

MBS142817

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Jun Proto-Oncogene

Jun Proto-Oncogene

C-JUN Human; Jun Proto-Oncogene Human Recombinant; Transcription factor AP-1; Activator protein 1; AP1; Proto-oncogene c-jun; V-jun avian sarcoma virus 17 oncogene homolog; p39; c-Jun

transcription factor AP-1; Transcription factor AP-1; transcription factor AP-1; Jun activation domain binding protein; activator protein 1; enhancer-binding protein AP1; jun oncogene; p39; proto-oncogene c-Jun; v-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog; jun proto-oncogene; Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39

C-JUN

JUN; JUN; AP1; AP-1; c-Jun; AP1

JUN_HUMAN

E Coli

331

Greater than 95% as determined by SDS-PAGE.

C-JUN is supplied as lyophilized powder containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Store at 4 degree C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Please note: Kinase activity may vary depending on the substrate and reaction conditions.

Solubility: It is recommended to centrifuge the vial prior to opening in order to bring the contents to the bottom. The reconstitution of the lyophilized c-Jun is recommended in 40mM Tris, pH 7.5, to a concentration of 0.2-1.0 mg/ml. Biological Activity: C-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.

PROTEIN KINASES; Enzymes; Jun Proto-Oncogene

Description: C-JUN amino acids 1-81 produced in E Coli, is a non-glycosylated, polypeptide chain having a molecular mass of 52 kDa.C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques. Introduction: C-JUN is a gene which, in combination with c-Fos, forms the AP-1early response transcription factor. C-JUN is activated by the JNKpathway. C-JUN is the putative transforming gene of avian sarcoma virus 17. C-JUN is a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. The C-JUN gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

4758616

NP_002219.1

NM_002228.3

P05412

35,676 Da

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (106400); Activation Of The AP-1 Family Of Transcription Factors Pathway (160139); AhR Pathway (755436); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546); Androgen Receptor Signaling Pathway (198806); Apoptosis Pathway (198797); Apoptosis Modulation And Signaling Pathway (198822)

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]

Jun: the protooncogene c-Jun is a component of the transcription factor AP-1, which binds and activates transcription at TRE/AP-1 elements. The Jun Kinases (JNKs) binds to the N-terminal region of c-Jun and phosphorylate it in response to extracellular signals including growth factors, cytokines and stress, regulating its transcriptional activity. Protein type: Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 1p32-p31. Cellular Component: nucleoplasm; nuclear chromosome; transcription factor complex; transcriptional repressor complex; nucleus; cytosol. Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; enzyme binding; DNA binding; transcription coactivator activity; double-stranded DNA binding; transcription factor binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; positive regulation of monocyte differentiation; response to cAMP; regulation of cell cycle; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; stress-activated MAPK cascade; microglial cell activation; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; SMAD protein nuclear translocation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; negative regulation of cell proliferation; response to radiation; positive regulation of fibroblast proliferation; regulation of transcription factor activity; transforming growth factor beta receptor signaling pathway; positive regulation of neuron apoptosis; negative regulation of protein amino acid autophosphorylation; angiogenesis; negative regulation of neuron apoptosis; toll-like receptor 4 signaling pathway; aging; response to drug; release of cytochrome c from mitochondria; monocyte differentiation; leading edge cell differentiation; MyD88-independent toll-like receptor signaling pathway; axon regeneration; learning; liver development; negative regulation of DNA binding; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; membrane depolarization; cellular response to potassium ion starvation; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of endothelial cell proliferation; toll-like receptor 9 signaling pathway; negative regulation of transcription, DNA-dependent; positive regulation of DNA replication

0.002 mg

140 USD

0.01 mg

205

1 mg

5595