protein

Recombinant Human Protein Kinase C Epsilon

MBS142816

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Protein Kinase C Epsilon

Kinase C Epsilon

PRKCE Human; Protein Kinase C epsilon Human Recombinant; Protein kinase C epsilon type; EC 2.7.11.13; nPKC-epsilon; PRKCE; PKCE; MGC125656; MGC125657

protein kinase C epsilon type; Protein kinase C epsilon type; protein kinase C epsilon type; protein kinase C, epsilon; nPKC-epsilon

PKC-e

PRKCE; PRKCE; PKCE; nPKC-epsilon; PKCE

KPCE_HUMAN

Sf9 Insect Cells

737

Greater than 90% as determined by SDS-PAGE.

PRKCE is supplied in 20mM Tris, pH 7.4, 0.1M NaCl, 20% glycerol, 1mM DTT, 0.1mM EDTA, 0.2mM PMSF, 1mM benzamidine and 0.03% Brij-35. Sterile Filtered clear solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Unit Defenition: 3250 units/mg. One unit of PRKCE activity transfers 1 nmole of phosphate to a PRKCE substrate peptide per minute at 30 degree C. Recombinant human active PRKCE also phosphorylates full length myelin basic protein, MARKS, GAP-34, and raf-1 kinase. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. The optimal concentration should be determined for each specific application.

PROTEIN KINASES; Enzymes; Protein Kinase-C

Description: PRKCE Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-737 and having a molecular mass of 87 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.PRKCE is purified by proprietary chromatographic techniques. Introduction: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.

4885563

NP_005391.1

NM_005400.2

Q02156

83,674 Da

B Cell Receptor Signaling Pathway (198909); CDC42 Signaling Events Pathway (137994); Calcium Regulation In The Cardiac Cell Pathway (198906); DAG And IP3 Signaling Pathway (477115); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Downstream Signaling Of Activated FGFR Pathway (160957)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior. [provided by RefSeq, Jul 2008]

PKCE: an AGC kinase of the PKC family. Calcium-independent, phospholipid-dependent. Activated by inflammatory mediators and involved in nociceptive functions. PKC-epsilon null mice display decreased hypoxic pulmonary vasoconstriction. Protein type: EC 2.7.11.13; Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Eta subfamily. Chromosomal Location of Human Ortholog: 2p21. Cellular Component: Golgi apparatus; cytoskeleton; mitochondrion; endoplasmic reticulum; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus; cytosol. Molecular Function: actin monomer binding; calcium-independent protein kinase C activity; protein serine/threonine kinase activity; signal transducer activity; protein binding; protein kinase C activity; enzyme binding; metal ion binding; enzyme activator activity; ethanol binding; ATP binding; receptor activator activity. Biological Process: nerve growth factor receptor signaling pathway; apoptosis; response to morphine; positive regulation of lipid catabolic process; signal transduction; protein amino acid phosphorylation; positive regulation of MAPKKK cascade; lipopolysaccharide-mediated signaling pathway; cell adhesion; regulation of peptidyl-tyrosine phosphorylation; positive regulation of cytokinesis; epidermal growth factor receptor signaling pathway; platelet activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; fibroblast growth factor receptor signaling pathway; positive regulation of synaptic transmission, GABAergic; positive regulation of insulin secretion; cell cycle; peptidyl-serine phosphorylation; positive regulation of actin filament polymerization; phospholipase C activation; cell division; release of sequestered calcium ion into cytosol; macrophage activation during immune response; innate immune response; blood coagulation

0.002 mg

140 USD

0.01 mg

205

1 mg

5595