Recombinant Human ELK-1 | MyBioSource MBS142803 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human ELK-1

catalog :

MBS142803

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142803

products type :

Recombinant Protein

products full name :

Recombinant Human ELK-1

products short name :

ELK-1

products name syn :

Elk-1 Human; Elk-1 Human Recombinant; ETS domain-containing protein Elk-1; ELK1; Elk-1

other names :

ETS domain-containing protein Elk-1 isoform a; ETS domain-containing protein Elk-1; ETS domain-containing protein Elk-1; ETS-like gene 1; tyrosine kinase (ELK1) oncogene; ELK1, member of ETS oncogene family

products gene name :

ELK-1

other gene names :

ELK1; ELK1

uniprot entry name :

ELK1_HUMAN

host :

E Coli

sequence length :

428

purity :

Greater than 95% as determined by SDS-PAGE.

form :

ELK-1 is supplied as lyophilized freeze dry powder without additives. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Store at 2-8 degree C if entire vial will be used within 1-2 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

other info1 :

Please note: Kinase activity may vary depending on the substrate and reaction conditions.

other info2 :

Biological Activity: Recombinant human Elk-1 is phosphorylatable in vitro, using either recombinant active ERK1/2, or with immunoprecipitated ERK1/2 from stimulated cells. This phosphorylation can be monitored by Western blot analysis. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application.

products categories :

PROTEIN KINASES; Enzymes; Protein Kinases

products description :

Description: ELK-1 (amino acids 307-428) Human Recombinant is produced in E Coli as a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and a c-terminal myc tag (EQKLISEEDL), and having a molecular mass of 60 kDa. Elk-1 is purified by proprietary chromatographic techniques. Introduction: Elk-1 is a transcription factor that is activated by mitogen-activated protein kinases (MAPKs) and whose main function is the regulation of growth-related proteins in response to extracellular stimuli. Because of the importance of Elk-1 to the regulation of successful cell growth, accurate monitoring of Elk-1 in cells, tissues and animals is crucial for many biomedical research and drug development projects.

ncbi gi num :

166362732

ncbi acc num :

NP_001107595.1

ncbi gb acc num :

NM_001114123.2

uniprot acc num :

P19419

ncbi mol weight :

11,217 Da

ncbi pathways :

Activated TLR4 Signalling Pathway (106400); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); B Cell Receptor Signaling Pathway (198909); BCR Signaling Pathway (138058); BDNF Signaling Pathway (712093); Corticotropin-releasing Hormone Pathway (920957); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); EGFR1 Signaling Pathway (198782); ERK/MAPK Targets Pathway (106482); Endometrial Cancer Pathway (83109)

ncbi summary :

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]

uniprot summary :

ELK1: a transcription factor of the Ets family and the ternary complex factor (TCF) subfamily. Forms a ternary complex by binding to the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. Mediates gene activity in response to serum and growth factors. Phosphorylated by MAP kinase pathways at a cluster of S/T motifs at its C-terminus; phosphorylation at these sites is critical for transcriptional activation by Elk-1. Two alternatively-spliced isoforms have been described. Protein type: DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: Xp11.2. Cellular Component: nucleoplasm; mitochondrion; cell soma; dendrite; nerve terminal; nucleus. Molecular Function: protein binding; double-stranded DNA binding; chromatin binding; transcription factor activity. Biological Process: transcription from RNA polymerase II promoter; response to light stimulus; nerve growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; positive regulation of transcription, DNA-dependent; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; cell differentiation; toll-like receptor 4 signaling pathway

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

6540

more info or order :

MyBioSource product webpage