protein

Recombinant Human TIE-2 Fc Chimera

MBS142794

0.002 mg

140 USD

Recombinant Protein

Recombinant Human TIE-2 Fc Chimera

TIE-2 Fc Chimera

TIE2 Fc Human; TIE2 Fc Chimera Human Recombinant; Angiopoietin-1 receptor precursor; Tyrosine-protein kinase receptor TIE-2; hTIE2; Tyrosine-protein kinase receptor TEK; p140 TEK; Tunica interna endothelial cell kinase; CD202b; VMCM; VMCM1; TIE2

angiopoietin-1 receptor isoform 1; Angiopoietin-1 receptor; angiopoietin-1 receptor; endothelial tyrosine kinase; tunica interna endothelial cell kinase; tyrosine kinase with Ig and EGF homology domains-2; tyrosine-protein kinase receptor TEK; tyrosine-protein kinase receptor TIE-2; TEK tyrosine kinase, endothelial; Endothelial tyrosine kinase; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; hTIE2; p140 TEK; CD_antigen: CD202b

TIE-2 Fc

TEK; TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B; TIE2; VMCM; VMCM1; hTIE2

TIE2_HUMAN

Insect Cells

1124

Greater than 90.0% as determined by(a)Analysis by RP-HPLC. (b)Analysis by SDS-PAGE.

TIE-2 Fc Chimera was lyophilized from a concentrated (1 mg/ml) sterile solution containing 1x PBS. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized sTIE-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution TIE-2 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized TIE-2 Fc Chimera in sterile water not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Measured in a functional ELISA assay. When TIE-2/Fc is immobilized at 4 ug/mL (100 ul/well), it binds rh Angiopoietin-2 with a linear range of 2 -100 ng/ml.

PROTEIN KINASES; Enzymes; TIE1,TIE2

Description: Soluble TIE-2 Human Recombinant fused with the Fc part of human IgG1 produced in baculovirus is a monomeric, glycosylated, polypeptide containing 730 amino acids and having a total molecular mass of 250 kDa. Human TIE-2/Fc monomer has a calculated molecular mass of approximately 125 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 140 kDa protein in SDS-PAGE under reducing conditions.The TIE2 Fc Chimera is purified by proprietary chromatographic techniques. Introduction: TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/Tek comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-1 cDNA encodes a 1122 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 726 residue extracellular domain and a 353 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high-affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or Tie-1 display similar angiogenic defects.

88758596

NP_000450.2

NM_000459.4

Q02763

109,141 Da

Angiogenesis Pathway (198772); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Cell Surface Interactions At The Vascular Wall Pathway (106062); HIF-1 Signaling Pathway (695200); Hemostasis Pathway (106028); PI3K-Akt Signaling Pathway (692234); PI3K-Akt Signaling Pathway (692979); Rap1 Signaling Pathway (868086); Rap1 Signaling Pathway (878042); Ras Signaling Pathway (868085)

This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Feb 2014]

TIE2: a receptor tyrosine kinase of the Tie family. Receptor for angiopoietin 1. Expressed almost exclusively in endothelial cells. May constitute the earliest mammalian endothelial cell lineage marker. Appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase. Protein type: Protein kinase, TK; EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); Membrane protein, integral; TK group; Tie family. Chromosomal Location of Human Ortholog: 9p21. Cellular Component: cell surface; focal adhesion; microvillus; integral to plasma membrane; basolateral plasma membrane; extracellular region; actin filament; intercellular junction; lipid raft; perinuclear region of cytoplasm; apical plasma membrane; stress fiber; basal plasma membrane; plasma membrane. Molecular Function: protein binding; protein-tyrosine kinase activity; growth factor binding; transmembrane receptor protein tyrosine kinase activity; receptor activity; ATP binding; protein kinase activity. Biological Process: response to peptide hormone stimulus; response to cAMP; peptidyl-tyrosine phosphorylation; heart development; cell-matrix adhesion; protein amino acid autophosphorylation; positive regulation of cytokine secretion during immune response; signal transduction; cell-cell adhesion; positive regulation of focal adhesion formation; cell-cell signaling; angiogenesis; endochondral ossification; organ regeneration; Tie receptor signaling pathway; regulation of establishment and/or maintenance of cell polarity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of peptidyl-serine phosphorylation; protein oligomerization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of angiogenesis; negative regulation of angiogenesis; positive regulation of protein import into nucleus; response to estrogen stimulus; negative regulation of inflammatory response; endothelial cell proliferation; response to hypoxia; positive regulation of endothelial cell proliferation; regulation of vascular permeability; sprouting angiogenesis; positive regulation of protein amino acid phosphorylation; blood coagulation; leukocyte migration; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis. Disease: Venous Malformations, Multiple Cutaneous And Mucosal

0.002 mg

140 USD

0.01 mg

205

1 mg

3680