protein

Recombinant Human Fibroblast Growth Factor Receptor-4

MBS142779

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Fibroblast Growth Factor Receptor-4

Fibroblast Growth Factor Receptor-4

FGFR4 Human; Fibroblast Growth Factor Receptor 4 Fc Chimera Human Recombinant; CD334; TKF; JTK2; FGFR4

fibroblast growth factor receptor 4 isoform 3; Fibroblast growth factor receptor 4; fibroblast growth factor receptor 4; hydroxyaryl-protein kinase; protein-tyrosine kinase; tyrosine kinase related to fibroblast growth factor receptor; tyrosylprotein kinase; fibroblast growth factor receptor 4; CD_antigen: CD334

FGFR4

FGFR4; FGFR4; TKF; JTK2; CD334; JTK2; TKF; FGFR-4

FGFR4_HUMAN

Insect Cells

734

Greater than 90.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

CD334 was lyophilized from a concentrated (1 mg/ml) sterile solution containing no additives. Sterile Filtered White lyophilized (freeze-dried) powder.

Lyophilized FGFR4 although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution FGFR4 should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized FGFR-4 in sterile PBS not less than 100 ug/ml, which can then be further diluted to other aqueous solutions. Biological Activity: Determined by its ability to inhibit human FGF acidic-dependent proliferation on R1 cells. The ED50 for this effect is typically at 15.0-30.0 ng/ml.

PROTEIN KINASES; Enzymes; FGF Receptors

Description: Soluble FGFR-4a (IIIc) Fc Chimera Human Recombinant fused with Xa cleavage site with the Fc part of human IgG1 produced in baculovirus is a heterodimeric, glycosylated, Polypeptide chain and having a molecular mass of 170 kDa. The FGFR4 is purified by proprietary chromatographic techniques. Introduction: Fibroblast growth factors (FGFs) comprise a family of at least eighteen structurally related proteins that are involved in a multitude of physiological and pathological cellular processes, including cell growth, differentiation, angiogenesis, wound healing and tumorgenesis. The biological activities of the FGFs are mediated by a family of type I transmembrane tyrosine kinases which undergo dimerization and autophosphorylation after ligand binding. Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs. A frequent splicing event involving FGF R1 and 2 results in receptors containing all three Ig domains, referred to as the a isoform, or only IgII and IgIII, referred to as the b isoform. Only the a isoform has been identified for FGF R3 and FGF R4. Additional splicing events for FGF R1 - 3, involving the C-terminal half of the IgIII domain encoded by two mutually exclusive alternative exons, generate FGF receptors with alternative IgIII domains (IIIb and IIIc). A IIIa isoform which is a secreted FGF binding protein containing only the N-terminal half of the IgIII domain plus some intron sequences has also been reported for FGF R1. Mutations in FGF R1 - 3 have been found in patients with birth defects involving craniosynostosis. The complex patterns of expression of these receptors as well as the specificity of their interactions with the various FGF ligand family members are under investigation.

634743314

NP_001278909.1

NM_001291980.1

P22455

64,640 Da

Adaptive Immune System Pathway 366160!!Constitutive PI3K/AKT Signaling In Cancer Pathway 685535!!DAP12 Interactions Pathway 685549!!DAP12 Signaling Pathway 685550!!Disease Pathway 530764!!Downstream Signal Transduction Pathway 106385!!Downstream Signaling Events Of B Cell Receptor (BCR) Pathway 576250!!Downstream Signaling Of Activated FGFR Pathway 160957!!Endocytosis Pathway 102279!!Endocytosis Pathway 102181

The protein encoded by this gene is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. The genomic organization of this gene, compared to members 1-3, encompasses 18 exons rather than 19 or 20. Although alternative splicing has been observed, there is no evidence that the C-terminal half of the IgIII domain of this protein varies between three alternate forms, as indicated for members 1-3. This particular family member preferentially binds acidic fibroblast growth factor and, although its specific function is unknown, it is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. [provided by RefSeq, Jul 2008]

0.01 mg

205 USD