protein

Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-6

MBS142767

0.005 mg

140 USD

Recombinant Protein

Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-6

Tyrosine Phosphatase Non Receptor Type-6

PTPN6 Human; Protein Tyrosine Phosphatase Non Receptor Type-6 Human Recombinant; Tyrosine-protein phosphatase non-receptor type 6; EC 3.1.3.48; Protein-tyrosine phosphatase 1C; PTP-1C; Hematopoietic cell protein-tyrosine phosphatase; SH-PTP1; Protein-tyrosine phosphatase SHP-1; PTPN6; HCP; HCPH; SHP1; HPTP1C; SHP-1L

tyrosine-protein phosphatase non-receptor type 6 isoform 1; Tyrosine-protein phosphatase non-receptor type 6; tyrosine-protein phosphatase non-receptor type 6; hematopoietic cell phosphatase; hematopoietic cell protein-tyrosine phosphatase; protein-tyrosine phosphatase 1C; protein-tyrosine phosphatase SHP-1; protein tyrosine phosphatase, non-receptor type 6; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; PTP-1C; Protein-tyrosine phosphatase SHP-1; SH-PTP1

PTPN6

PTPN6; PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1; HCP; PTP1C; PTP-1C

PTN6_HUMAN

E Coli

595

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Greater than 95.0% as determined by SDS-PAGE.

The protein contains 25mM Tris-HCl, pH 7.5, 2mM b-mercaptoethanol, 1mM EDTA, 1mMDTT and 20%Glycerol. Sterile filtered colorless solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Unit Defenition: One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate per minute at pH 7.5 at 37 degree C using 10mM of substrate.

Activity: >5,000 U/mg of PTPN6.

PROTEIN KINASES; Enzymes; Phosphatase

Description: PTPN6 Human Recombinant produced in E Coli is a single,non-glycosylated polypeptide chain containing 300 amino acids and having a molecular mass of 34.3 kDa. The protein coding region of the catalytic domain of PTPN6 (amino acids 243-541). The catalytic domain of PTPN6 was overexpressed as insoluble protein aggregates (inclusion bodies). The recombinant PTPN6 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer. Additional amino acid(Met) is attached at N-terminus. Introduction: PTPN6 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported.

18104989

NP_002822.2

NM_002831.5

P29350

70,131 Da

Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); B Cell Receptor Signaling Pathway (198909); B Cell Receptor Signaling Pathway (83081); B Cell Receptor Signaling Pathway (492); BCR Signaling Pathway (138058); CXCR4-mediated Signaling Events Pathway (137910); Cell Surface Interactions At The Vascular Wall Pathway (106062); Cell-Cell Communication Pathway (477132)

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of this PTP contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of this PTP with its substrates. This PTP is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. This PTP has been shown to interact with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling. Multiple alternatively spliced variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq, Jul 2008]

SHP-1: an SH2-containing ubiquitously expressed tyrosine-specific protein phosphatase. Plays a key role in hematopoiesis. Directly links growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Three alternatively spliced isoforms have been described. Protein type: Motility/polarity/chemotaxis; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48. Chromosomal Location of Human Ortholog: 12p13. Cellular Component: membrane; cytoplasm; nucleolus; alpha-beta T cell receptor complex; intercellular junction; nucleus; cytosol. Molecular Function: protein binding; phosphotyrosine binding; transmembrane receptor protein tyrosine phosphatase activity; protein tyrosine phosphatase activity; SH2 domain binding; protein kinase binding; SH3 domain binding. Biological Process: negative regulation of MAP kinase activity; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid dephosphorylation; negative regulation of humoral immune response mediated by circulating immunoglobulin; negative regulation of cell proliferation; B cell receptor signaling pathway; negative regulation of T cell proliferation; negative regulation of B cell receptor signaling pathway; positive regulation of cell proliferation; abortive mitotic cell cycle; cell differentiation; negative regulation of peptidyl-tyrosine phosphorylation; natural killer cell mediated cytotoxicity; cytokine and chemokine mediated signaling pathway; negative regulation of T cell receptor signaling pathway; positive regulation of cell adhesion mediated by integrin; regulation of release of sequestered calcium ion into cytosol; positive regulation of phosphoinositide 3-kinase cascade; cell proliferation; G-protein coupled receptor protein signaling pathway; T cell costimulation; regulation of B cell differentiation; platelet formation; blood coagulation; leukocyte migration

0.005 mg

140 USD

0.02 mg

205

1 mg

2750