Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-1 | MyBioSource MBS142765 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-1

catalog :

MBS142765

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142765

products type :

Recombinant Protein

products full name :

Recombinant Human Protein Tyrosine Phosphatase Non Receptor Type-1

products short name :

Tyrosine Phosphatase Non Receptor Type-1

products name syn :

PTPN1 Human; Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant; Tyrosine-protein phosphatase non-receptor type 1; EC 3.1.3.48; Protein-tyrosine phosphatase 1B; PTP-1B; PTPN1; PTP1B

other names :

tyrosine-protein phosphatase non-receptor type 1 isoform 2; Tyrosine-protein phosphatase non-receptor type 1; tyrosine-protein phosphatase non-receptor type 1; protein tyrosine phosphatase, placental; protein-tyrosine phosphatase 1B; protein tyrosine phosphatase, non-receptor type 1; Protein-tyrosine phosphatase 1B; PTP-1B

products gene name :

PTPN1

other gene names :

PTPN1; PTPN1; PTP1B; PTP1B; PTP-1B

uniprot entry name :

PTN1_HUMAN

host :

E Coli

sequence length :

362

sequence :

memekefeqi dksgswaaiy qdirheasdf pcrvaklpkn knrnryrdvs pfdhsriklhqedndyinas likmeeaqrs yiltqgplpn tcghfwemvw eqksrgvvml nrvmekgslkcaqywpqkee kemifedtnl kltlisedik syytvrqlel enlttqetre ilhfhyttwpdfgvpespas flnflfkvre sgslspehgp vvvhcsagig rsgtfcladt clllmdkrkdpssvdikkvl lemrkfrmgl iqtadqlrfs ylaviegakf imgdssvqdq wkelshedle pppehipppprppkrilephn.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The protein contains 25mM Tris-HCl, pH 7.5, 2mM b-mercaptoethanol, 1mM EDTA, 1mM DTT and 20% Glycerol. Sterile filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

other info1 :

Unit Defenition: One unit will hydrolyze 1 nanomole of p-nitrophenylphosphatate per minute at pH 7.4 at 37 degree C using 10mM of substrate.

other info2 :

Biological Activity: Specific activity: 6,000-12,000units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme hydrolyzing 1 nmole of p-nitrophenyl phosphate (pNPP) per minute at 37C, pH7.5 using 10mM of substrate.

products categories :

PROTEIN KINASES; Enzymes; Phosphatase

products description :

Description: Protein Tyrosine Phosphatase Non Receptor Type-1 Human Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 321 amino acids and having a molecular mass of 37.3 kDa. Introduction: Protein Tyrosine Phosphatase 1B is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.

ncbi gi num :

519666804

ncbi acc num :

NP_001265547.1

ncbi gb acc num :

NM_001278618.1

uniprot acc num :

P18031

ncbi mol weight :

49,967 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway (137993); Cytokine Signaling In Immune System Pathway (366171); EGF Receptor (ErbB1) Signaling Pathway (137970); Growth Hormone Receptor Signaling Pathway (477128); Hemostasis Pathway (106028); IGF1 Pathway (137992); Immune System Pathway (106386); Insulin Pathway (138026)

ncbi summary :

The protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2013]

uniprot summary :

PTP1B: a non-receptor phospho-tyrosine protein phosphatase which acts as a regulator of endoplasmic reticulum unfolded protein response. Mediates dephosphorylation and inactivation of PERK. May play an important role in CKII- and p60c-src-induced signal transduction cascades. May regulate the EFNA5-EPHA3 signaling pathway which modulates cell reorganization and cell-cell repulsion. Negatively regulates insulin signaling by dephosphorylating the phosphotyrosine residues of insulin receptor. Also reported to dephosphorylate integrin, epidermal growth factor receptor, JAK2 and TYK2, regulating cell growth control and the cellular response to interferon. May regulate the hepatocyte growth factor receptor signaling pathway through dephosphorylation of MET. PTP1B knockout mice show resistance to dietary weight gain and enhanced insulin sensitivity, suggesting a possible role in treatment of obesity as well as type 2 diabetes. Protein type: Motility/polarity/chemotaxis; Phosphatase; Protein phosphatase, tyrosine (non-receptor); EC 3.1.3.48. Chromosomal Location of Human Ortholog: 20q13.1-q13.2. Cellular Component: endoplasmic reticulum; early endosome; plasma membrane; cytoplasmic vesicle; cytosol. Molecular Function: protein binding; enzyme binding; ephrin receptor binding; zinc ion binding; protein tyrosine phosphatase activity; receptor tyrosine kinase binding; protein kinase binding; insulin receptor binding. Biological Process: platelet activation; unfolded protein response, activation of signaling protein activity; negative regulation of vascular endothelial growth factor receptor signaling pathway; unfolded protein response; cytokine and chemokine mediated signaling pathway; regulation of endocytosis; actin cytoskeleton reorganization; insulin receptor signaling pathway; negative regulation of insulin receptor signaling pathway; regulation of signal transduction; blood coagulation. Disease: Diabetes Mellitus, Noninsulin-dependent

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

205

size3 :

1 mg

price3 :

1790

more info or order :

MyBioSource product webpage