protein

Recombinant Human Protein Kinase C alpha

MBS142764

0.002 mg

140 USD

Recombinant Protein

Recombinant Human Protein Kinase C alpha

Kinase C alpha

PKC-a Human; Protein Kinase C alpha Human Recombinant; Protein kinase C alpha type; EC 2.7.11.13; PKC-alpha; PKC-A; PRKCA; AAG6; PKCA; PRKACA; MGC129900; MGC129901

protein kinase C alpha type; Protein kinase C alpha type; protein kinase C alpha type; PKC-A; aging-associated gene 6; protein kinase C, alpha

PKC-a

PRKCA; PRKCA; AAG6; PKCA; PRKACA; PKC-alpha; PKCA; PRKACA; PKC-A; PKC-alpha

KPCA_HUMAN

Sf9 Insect Cells

672

MADVYPGNDS TASQDVANRF ARKGALRQKN VHEVKDHKFI ARFFKQPTFC SHCTDFIWGF GKQGFQCQVC CFVVHKRCHE FVTFSCPGAD KGPDTDDPRS KHKFKIHTYG SPTFCDHCGS LLYGLIHQGM KCDTCDMNVH KQCVINVPSL CGMDHTEKRG RIYLKAEVAD EKLHVTVRDA KNLIPMDPNG LSDPYVKLKL IPDPKNESKQ KTKTIRSTLN PQWNESFTFK LKPSDKDRRL SVEIWDWDRT TRNDFMGSLS FGVSELMKMP ASGWYKLLNQ EEGEYYNVPI PEGDEEGNME LRQKFEKAKL GPAGNKVISP SEDRKQPSNN LDRVKLTDFN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLDNVM LDSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSICK GLMTKHPAKR LGCGPEGERD VREHAFFRGI DWEKLENREI QPPFKPKVCG KGAENFDKFF TRGQPVLTPP DQLVIANIDQ SDFEGFSYVN PQFVHPILQS AV.

Greater than 95% as determined by SDS-PAGE.

Dilution Buffer: 10mM HEPES (pH7.4), 0.01% CHAPS and 5mM DTT.*for further dilution you may use either the Storage Buffer or Dilution Buffer. Unit Defenition: 7200- 9000 units/mg. One unit of PKC-a activity transfers 1 nmole of phosphate to a PKC substrate peptide (myelin basic protein 4-14) per minute at 30 degree C. Recombinant human active PKC-a also phosphorylates full length myelin basic protein, histone, MARKS, and GSK-3b. Activity requires incubation with lipid activators, and may vary depending on the substrate and reaction conditions. Storage Buffer: PKC-a is supplied at a of 0.37mg/ml in 20mM HEPES pH 7.5, 250mM NaCl, 50% glycerol, 2mM EDTA,2mM EGTA, 0.05%Triton X-100 and 5mM DTT. Specific Activity: 1570 nmole of phosphate transferred to Histone H1 substrate per minute per mg of total protein at 30 degree C. Activity determined at a final protein concentration of 0.5 to 2ug/ml.

PROTEIN KINASES; Enzymes; Protein Kinase-C

Description: Protein Kinase C alpha Human Recombinant produced in Sf9 is a glycosylated, polypeptide chain containing amino acids 2-672 and having a molecular mass of 76.7 KD. This protein is the full-length form of the protein with an amino terminal poly His-tag.PKC-a is purified by proprietary chromatographic techniques. Introduction: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes.

4506067

NP_002728.1

NM_002737.2

P17252

76,750 Da

AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Acetylcholine Regulates Insulin Secretion Pathway (105924); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); African Trypanosomiasis Pathway (194384); African Trypanosomiasis Pathway (194323); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Alpha6-Beta4 Integrin Signaling Pathway (198807)

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators. Protein type: Oncoprotein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Protein kinase, AGC; Kinase, protein; AGC group; PKC family; Alpha subfamily. Chromosomal Location of Human Ortholog: 17q22-q23.2. Cellular Component: nucleoplasm; photoreceptor outer segment; cell soma; mitochondrion; perinuclear region of cytoplasm; apical part of cell; endoplasmic reticulum; dendrite; cytoplasm; mitochondrial membrane; plasma membrane; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; enzyme binding; zinc ion binding; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity. Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; regulation of muscle contraction; positive regulation of lipopolysaccharide-mediated signaling pathway; mitotic nuclear envelope disassembly; negative regulation of insulin receptor signaling pathway; positive regulation of mitotic cell cycle; signal transduction; protein amino acid phosphorylation; induction of positive chemotaxis; negative regulation of cell proliferation; synaptic transmission; chondrocyte differentiation; angiogenesis; cell adhesion; positive regulation of macrophage differentiation; regulation of peptidyl-tyrosine phosphorylation; regulation of the force of heart contraction; inactivation of MAPK activity; epidermal growth factor receptor signaling pathway; neutrophil chemotaxis; platelet activation; fibroblast growth factor receptor signaling pathway; positive regulation of blood vessel endothelial cell migration; negative regulation of adenylate cyclase activity; adenylate cyclase activation; cellular calcium ion homeostasis; rhodopsin mediated signaling; negative regulation of glucose import; positive regulation of angiogenesis; regulation of rhodopsin mediated signaling; phospholipase C activation; energy reserve metabolic process; innate immune response; gene expression; positive regulation of endothelial cell proliferation; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; blood coagulation; regulation of insulin secretion; positive regulation of inflammatory response; positive regulation of cell migration

0.002 mg

140 USD

0.01 mg

205

0.1 mg

1125