protein

Recombinant Human p38 alpha/SAPK2 alpha

MBS142763

0.001 mg

205 USD

Recombinant Protein

Recombinant Human p38 alpha/SAPK2 alpha

p38 alpha/SAPK2 alpha

p38a/SAPK2 Human; p38a/SAPK2 Human Recombinant; Mitogen-activated protein kinase 14; EC 2.7.11.24; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAX-interacting protein 2; MAP kinase MXI2; SAPK2A; RK; p38; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; p38ALPHA

mitogen-activated protein kinase 14 isoform 1; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 14; CSAID-binding protein; Csaids binding protein; MAP kinase 14; MAP kinase Mxi2; MAP kinase p38 alpha; MAX-interacting protein 2; cytokine suppressive anti-inflammatory drug binding protein; cytokine suppressive anti-inflammatory drug-binding protein; mitogen-activated protein kinase p38 alpha; p38 MAP kinase; p38 mitogen activated protein kinase; p38alpha Exip; stress-activated protein kinase 2A; mitogen-activated protein kinase 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a

p38a/SAPK2a

MAPK14; MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA; CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; MAP kinase 14; MAPK 14; CSAID-binding protein; CSBP; MAP kinase p38 alpha; SAPK2a

MK14_HUMAN

E Coli

360

Greater than 95% as determined by SDS-PAGE.

p38/SAPK2 is supplied 1.19mg/ml in 25mM Tris-HCl, 150mM NaCl, 1mM DTT, 50% glycerol, pH 8.5. Sterile Filtered clear solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

Unit Defenition: No protease activity detectable, specific activity is 2.357.900 U*/mg (*1 U = 1 pmol/min transferred to myelin basic protein at 30 degree C).

PROTEIN KINASES; Enzymes; Protein Kinases

Description: p38/SAPK2 is a non-glycosilated polypeptide produced by phosphorylation of the purified p38 alpha with MKK6 having a molecular mass of 42.7 kDa. Introduction: p38a/SAPK2 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

4503069

NP_001306.1

NM_001315.2

Q16539

29,388 Da

ADP Signalling Through P2Y Purinoceptor 1 Pathway (161061); AGE/RAGE Pathway (698754); ATF-2 Transcription Factor Network Pathway (138006); Activated TLR4 Signalling Pathway (106400); Activation Of PPARGC1A (PGC-1alpha) By Phosphorylation Pathway (1127496); Activation Of The AP-1 Family Of Transcription Factors Pathway (160139); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099)

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on Ser-10 (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Protein type: EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/p38 subfamily; p38 subfamily. Chromosomal Location of Human Ortholog: 6p21.3-p21.2. Cellular Component: nucleoplasm; spindle pole; mitochondrion; cytoplasm; nucleus; cytosol. Molecular Function: MAP kinase activity; MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; NFAT protein binding; ATP binding. Biological Process: nerve growth factor receptor signaling pathway; apoptosis; activation of MAPK activity; stress-activated MAPK cascade; cell morphogenesis; toll-like receptor 3 signaling pathway; osteoclast differentiation; chemotaxis; signal transduction; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; cell surface receptor linked signal transduction; regulation of transcription factor activity; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; chondrocyte differentiation; angiogenesis; toll-like receptor 4 signaling pathway; cartilage condensation; platelet activation; mitochondrion organization and biogenesis; skeletal muscle development; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; MyD88-independent toll-like receptor signaling pathway; organelle organization and biogenesis; positive regulation of blood vessel endothelial cell migration; DNA damage response, signal transduction; glucose metabolic process; positive regulation of myoblast differentiation; toll-like receptor 2 signaling pathway; regulation of transcription from RNA polymerase II promoter; MyD88-dependent toll-like receptor signaling pathway; muscle cell differentiation; peptidyl-serine phosphorylation; positive regulation of protein import into nucleus; response to muramyl dipeptide; DNA damage checkpoint; Ras protein signal transduction; toll-like receptor signaling pathway; positive regulation of muscle cell differentiation; striated muscle cell differentiation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; gene expression; toll-like receptor 9 signaling pathway; cell motility; vascular endothelial growth factor receptor signaling pathway; blood coagulation; myoblast cell differentiation involved in skeletal muscle regeneration

0.001 mg

205 USD

0.005 mg

455

0.02 mg

1075