Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha | MyBioSource MBS142746 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha

catalog :

MBS142746

quantity :

0.001 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142746

products type :

Recombinant Protein

products full name :

Recombinant c-AMP dependant Protein Kinase A catalytic subunit alpha

products short name :

c-AMP dependant Protein Kinase A catalytic subunit alpha

products name syn :

PRKACA; cAMP-Dependent Protein Kinase A catalytic subunit alpha Recombinant; cAMP-dependent protein kinase alpha-catalytic subunit; EC 2.7.11.11; PKA C-alpha; PKACA; PRKACA; MGC48865; MGC102831

other names :

cAMP-dependent protein kinase catalytic subunit alpha isoform Calpha1; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha

products gene name :

PRKACA

other gene names :

PRKACA; PRKACA; PKACA; PPNAD4; PKACA; PKA C-alpha

uniprot entry name :

KAPCA_HUMAN

host :

E Coli

sequence length :

351

purity :

Greater than 95% as determined by SDS-PAGE.

form :

PKA catalytic subunit a is supplied in a buffer containing 25mM potassium phosphate (pH 6.5), 5mM 2-mercaptoethanol, 5mM EDTA, 150mM NaCl and 50% glycerol.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

other info1 :

Unit Defenition: One unit is defined as the amount of recombinant PKA catalytic subunit a, required to incorporate 1pmol of phosphate into the specific substrate peptide kemptide (LRRASlG) in one minute at 30 degree C. Specific Activity: The specific activity of the recombinant PKA catalytic subunit a, is >15.000.000 U/mg.

products categories :

PROTEIN KINASES; Enzymes; Protein Kinase-A

products description :

Description: cAMP-dependent PKA is an ubiquitous serine/threonine protein kinase present in a variety of tissues (e.g. brain, skeletal muscle, heart). The intracellular cAMP level regulates cellular responses by altering the interaction between the catalytic C and regulatory R subunits of PKA. The inactive tetrameric PKA holoenzyme R2C2 is activated when cAMP binds to R2, which dissociates the tetramer to R2 cAMP 4 and two active catalytic subunits. Free Catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins. In response to hormone- induced high cAMP levels, PKA phosphorylates glycogen synthetase (inhibition of the enzyme activity) and phosphorylase kinase to block glycogen synthesis. Different isoforms of catalytic and regulatory subunits suggest specific functions. The recombinant PKA catalytic subunit a is a 41kDa protein. The a-isoform is the predominant form with a broad tissue distribution and can be used for in vitro enzymological studies of neural and hormonal signal transduction or to phosphorylate target proteins in vivo including Ion channels, transcriptional activator proteins and regulatory enzymes of glycogen metabolism.

ncbi gi num :

4506055

ncbi acc num :

NP_002721.1

ncbi gb acc num :

NM_002730.3

uniprot acc num :

P17612

ncbi mol weight :

39,822 Da

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Adaptive Immune System Pathway (366160); Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Alcoholism Pathway (585563); Alcoholism Pathway (587116); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546)

ncbi summary :

This gene encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits, in its inactive form. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of this gene caused either by somatic mutations, or genomic duplications of regions that include this gene, have been associated with hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. Tissue-specific isoforms that differ at the N-terminus have been described, and these isoforms may differ in the post-translational modifications that occur at the N-terminus of some isoforms. [provided by RefSeq, Jan 2015]

uniprot summary :

PKACA: catalytic subunit of cAMP-dependent protein kinase alpha, an AGC kinase. A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Two splice-variant isoforms have been described. Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.11; AGC group; PKA family. Chromosomal Location of Human Ortholog: 19p13.1. Cellular Component: centrosome; mitochondrion; membrane; plasma membrane; nucleus; neuromuscular junction; cytosol; cAMP-dependent protein kinase complex; AMP-activated protein kinase complex. Molecular Function: protein binding; cAMP-dependent protein kinase activity; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; protein kinase binding; ATP binding. Biological Process: nerve growth factor receptor signaling pathway; water transport; protein amino acid autophosphorylation; pathogenesis; signal transduction; protein amino acid phosphorylation; positive regulation of protein export from nucleus; triacylglycerol catabolic process; G2/M transition of mitotic cell cycle; transmembrane transport; sperm capacitation; epidermal growth factor receptor signaling pathway; regulation of synaptic transmission; cytosolic calcium ion homeostasis; fibroblast growth factor receptor signaling pathway; regulation of osteoblast differentiation; regulation of heart rate; organelle organization and biogenesis; activation of protein kinase A; peptidyl-threonine phosphorylation; glucose metabolic process; gluconeogenesis; peptidyl-serine phosphorylation; mesoderm formation; phospholipase C activation; regulation of protein binding; carbohydrate metabolic process; neural tube closure; energy reserve metabolic process; renal water homeostasis; innate immune response; mitotic cell cycle; blood coagulation; regulation of insulin secretion

size1 :

0.001 mg

price1 :

140 USD

size2 :

0.005 mg

price2 :

205

size3 :

0.1 mg

price3 :

2115

more info or order :

MyBioSource product webpage