catalog number :
MBS142701
products type :
Recombinant Protein
products full name :
Recombinant E Coli Dnak Lid Covering Substrate
products short name :
Dnak Lid Covering Substrate
products name syn :
DnaK Lid Covering Substrate E.Coli; DnaK Lid Covering Substrate E.Coli Recombinant; HSP-70; HSP70; DnaK; Chaperone protein dnaK; Heat shock protein 70; Heat shock 70 kDa protein; groP; grpF; seg; b0014; JW0013; Dnak LCS
other names :
DnaK; Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70
products gene name :
Dnak
uniprot entry name :
DNAK_VIBPR
sequence :
MNEDEIQKMV RDAEANAEAD RKFEELVQTR NQGDHLLHST RKQVEEAGDK LPADDKTAIESALTALETAL KGEDKAAIEA KMQELAQVSQ KLMEIAQQQH AQQQTAGADASANNAKDDDVVDAEFEEVKDKK.
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT and 10%Glycerol. Sterile filtered colorless solution.
storage stability :
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
products categories :
HEAT SHOCK PROTEINS; Recombinant Proteins; Heat Shock Protein
products description :
Description: Recombinant DnaK Lid Covering Substrate domain produced in E Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids and having a molecular mass of 14.6 kDa. Introduction: DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins. Dnak(residues 508-638) of the substrate binding domain is a-helical and appears to act as a lid covering the substrate binding cleft. DnaK(amino acid 508-638) was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
ncbi acc num :
AAF27648.1
ncbi mol weight :
68,987 Da