catalog number :
MBS142699
products type :
Recombinant Protein
products full name :
Recombinant E Coli Dnak Substrate Binding Domain C-terminal
products short name :
Dnak Substrate Binding Domain C-terminal
products name syn :
Dnak SBD C-terminus E.Coli; Dnak Substrate Binding Domain C-terminal E.Coli Recombinant; HSP-70; HSP70; DnaK; Chaperone protein dnaK; Heat shock protein 70; Heat shock 70 kDa protein; groP; grpF; seg; b0014; JW0013; Dnak SBD C-terminal
other names :
DnaK; Chaperone protein DnaK; HSP70; Heat shock 70 kDa protein; Heat shock protein 70
products gene name :
Dnak
uniprot entry name :
DNAK_VIBPR
sequence :
MDVKDVLLLD VTPLSLGIET MGGVMTTLIA KNTTIPTKHS QVFSTAEDNQ SAVTIHVLQGERKRAADNKS LGQFNLDGIN PAPRGMPQIE VTFDIDADGI LHVSAKDKNS GKEQKITIKA SSGLNEDEIQ KMVRDAEANA EADRKFEELV QTRNQGDHLL HSTRKQVEEA GDKLPADDKTAIESALTALE TALKGEDKAA IEAKMQELAQ VSQKLMEIAQ QQHAQQQTAG ADASANNAKD DDVVDAEFEE VKDKK.
purity :
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
The DnaK protein contains 25mM Tris-HCl, pH7.5, 100mM NaCl, 5mM DTT and 10%Glycerol. Sterile filtered colorless solution.
storage stability :
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
products categories :
HEAT SHOCK PROTEINS; Recombinant Proteins; Heat Shock Protein
products description :
Description: Recombinant Dnak Substrate Binding Domain C-terminal produced in E Coli is a single, non-glycosylated polypeptide chain containing 255 amino acids and having a molecular mass of 27.7 kDa. Introduction: DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial hsp70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.The protein coding region of the substrate binding domain of DnaK (amino acids 385-638) was amplified by PCR and cloned into an E. coli expression vector. The substrate binding domain of DNAK was purified to apparent homogeneity by using conventional column chromatography techniques. Additional amino acid (Met) is attached at N- terminus.
ncbi acc num :
AAF27648.1
ncbi mol weight :
68,987 Da