catalog number :
MBS142697
products type :
Recombinant Protein
products full name :
Recombinant E Coli DnaJ (HSP40)
products short name :
DnaJ
products name syn :
DnaJ E.Coli; DnaJ (HSP40) E.Coli Recombinant; HSP-40; HSP40; DnaJ; DNAJB1; HSPF1; Hdj1; Chaperone protein dnaJ; Heat shock protein J; groP; b0015; JW0014
other names :
chaperone Hsp40, DnaK co-chaperone; Chaperone protein DnaJ; chaperone Hsp40, DnaK co-chaperone; HSP40; Heat shock protein J
products gene name :
DnaJ
other gene names :
dnaJ; dnaJ; ECK0015; faa; groP; grpC; JW0014; groP
uniprot entry name :
DNAJ_ECOLI
sequence :
MAKQDYYEIL GVSKTAEEHE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDSQKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYNMELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQQTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAPAGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRSKSFFDGVKKF FDDLTR.
purity :
Greater than 95.0% as determined by SDS-PAGE.
form :
The DnaJ contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 5mM DTT and 10% Glycerol. Sterile filtered colorless solution.
storage stability :
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
products categories :
HEAT SHOCK PROTEINS; Recombinant Proteins; Heat Shock Protein
products description :
Description: Recombinant Dna-J produced in E Coli is a single, non-glycosylated polypeptide chain containing 376 amino acids and having a molecular mass of 41.1 kDa. Introduction: DnaJ, Heat shock protein, functions in association with DnaK(Hsp70) molecular chaperone to facilitate protein folding. p70 chaperone. DnaJ plays a key role in the chaperone reaction by stimulating the ATPase activity and activating the substrate binding of Hsp70. DnaJ consists of four domains that are N-terminal 76 amino acid J-domain, G/F domain, zinc-binding cystein rich CR-domain, C-terminal CTD-domain and they are conserved to various degrees among the homologues.
ncbi acc num :
NP_414556.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
41,100 Da
ncbi summary :
DnaK and DnaJ both bind to the same target peptide, forming a ternary complex. [More information is available at EcoGene: EG10240]. The DnaK system of Escherichia coli is a homolog of the eukaryotic Hsp70 chaperone system. [More information is available at EcoCyc: EG10240].