catalog number :
MBS142696
products type :
Recombinant Protein
products full name :
Recombinant E Coli Dnak (HSP70)
products short name :
Dnak
products name syn :
DnaK E.coli; DnaK (HSP70) E.Coli Recombinant; HSP-70; HSP70; DnaK; Chaperone protein dnaK; Heat shock protein 70; Heat shock 70 kDa protein; groP; grpF; seg; b0014; JW0013
other names :
chaperone Hsp70, with co-chaperone DnaJ; Chaperone protein DnaK; chaperone Hsp70, with co-chaperone DnaJ; HSP70; Heat shock 70 kDa protein; Heat shock protein 70
products gene name :
Dnak
other gene names :
dnaK; dnaK; ECK0014; groPAB; groPC; groPF; grpC; grpF; JW0013; seg; groP; grpF; seg
uniprot entry name :
DNAK_ECOLI
sequence :
MGKIIGIDLG TTNSCVAIMD GTTPRVLENA EGDRTTPSII AYTQDGETLV GQPAKRQAVTNPQNTLFAIK RLIGRRFQDE EVQRDVSIMP FKIIAADNGD AWVEVKGQKM APPQISAEVLKKMKKTAEDY LGEPVTEAVI TVPAYFNDAQ RQATKDAGRI AGLEVKRIINEPTAAALAYGLDKGTGNRTI AVYDLGGGTFDISIIEIDEV DGEKTFEVLA TNGDTHLGGE DFDSRLINYLVEEFKKDQGI DLRNDPLAMQ RLKEAAEKAK IELSSAQQTD VNLPYITADA TGPKHMNIKVTRAKLESLVE DLVNRSIEPL KVALQDAGLS VSDIDDVILV GGQTRMPMVQ KKVAEFFGKEPRKDVNPDEA VAIGAAVQGG VLTGDVKDVL LLDVTPLSLG IETMGGVMTT LIAKNTTIPTKHSQVFSTAE DNQSAVTIHV LQGERKRAAD NKSLGQFNLD GINPAPRGMPQIEVTFDIDADGILHVSAKD KNSGKEQKITIKASSGLNED EIQKMVRDAE ANAEADRKFE ELVQTRNQGDHLLHSTRKQV EEAGDKLPAD DKTAIESALT ALETALKGED KAAIEAKMQE LAQVSQKLMEIAQQQHAQQQ TAGADASANN AKDDDVVDAE FEEVKDKK.
purity :
Greater than 95.0% as determined by SDS-PAGE.
form :
The DnaK protein contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 5mM DTT and 10% Glycerol. Sterile filtered colorless solution.
storage stability :
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
products categories :
HEAT SHOCK PROTEINS; Recombinant Proteins; Heat Shock Protein
products description :
Description: Recombinant Dnak produced in E Coli is a single, non-glycosylated polypeptide chain containing 638 amino acids and having a molecular mass of 69 kDa. Introduction: DnaK, originally identified for its DNA replication by bacteriophage l in E. coli is the bacterial HSP70 chaperone. This protein is involved in the folding and assembly of newly synthesized polypeptide chains and in preventing the aggregation of stress-denatured proteins.
ncbi acc num :
NP_414555.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
69,115 Da
ncbi pathways :
RNA Degradation Pathway (116132); RNA Degradation Pathway (116127)
ncbi summary :
ATP-regulated binding and release of polypeptide substrates. [More information is available at EcoGene: EG10241]. Hsc56 exhibits specificity toward Hsc62, as Hsc56 does not activate DnaK or Hsc66 ATPase activity . [More information is available at EcoCyc: EG10241].