catalog number :
MBS142694
products type :
Recombinant Protein
products full name :
Recombinant E Coli GroEL (HSP60)
products short name :
GroEL
products name syn :
GroEL E.Coli; GroEL (HSP60) E.Coli Recombinant; CPN60; GROEL; HSP60; HSP65; SPG13; CHA60; GROL; crpA; mopA; 60 kDa chaperonin; Protein Cpn60; groEL protein; b4143; JW4103
other names :
Cpn60 chaperonin GroEL, large subunit of GroESL; 60 kDa chaperonin; Cpn60 chaperonin GroEL, large subunit of GroESL; GroEL protein; Protein Cpn60
products gene name :
GROEL
other gene names :
groL; groL; ECK4137; groEL; JW4103; mopA
uniprot entry name :
CH60_ECOLI
sequence :
MAAKDVKFGN DARVKMLRGV NVLADAVKVT LGPKGRNVVL DKSFGAPTIT KDGVSVAREIELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQAII TEGLKAVAAG MNPMDLKRGIDKAVTAAVEE LKALSVPCSD SKAIAQVGTI SANSDETVGK LIAEAMDKVG KEGVITVEDGTGLQDELDVV EGMQFDRGYL SPYFINKPET GAVELESPFI LLADKKISNI REMLPVLEAVAKAGKPLLII AEDVEGEALA TAVVNTIRGI VKVAAVKAPG FGDRRKAMLQ DIATLTGGTVISEEIGMELE KATLEDLGQA KRVVINKDTT TIIDGVGEEA AIQGRVAQIR QQIEEATSDYDREKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALIRVASKLADLR GQNEDQNVGI KVALRAMEAP LRQIVLNCGE EPSVVANTVK GGDGNYGYNAATEEYGNMID MGILDPTKVT RSALQYAASV AGLMITTECM VTDLPKNDAA DLGAAGGMGG MGGMGGMM.
purity :
Greater than 95.0% as determined by SDS-PAGE.
form :
The groEL protein contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 1mM DTT and 10% Glycerol. Sterile filtered colorless solution.
storage stability :
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
products categories :
HEAT SHOCK PROTEINS; Recombinant Proteins; Heat Shock Protein
products description :
Description: Recombinant GroEL produced in E Coli is a single, non-glycosylated polypeptide chain containing 548 amino acids and having a molecular mass of 57.3kDa. Introduction: GroEL protein is the major heat shock protein of E.coli and belongs to the chaperonin (HSP60) family. GroEL protein prevents misfolding of proteins and promotes the refolding and proper assembly of unfolded polypeptiedes generated under stress condition.
ncbi acc num :
NP_418567.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
57,329 Da
ncbi pathways :
RNA Degradation Pathway (116132); RNA Degradation Pathway (116127)
ncbi summary :
groL is an essential gene. [More information is available at EcoGene: EG10599]. The Escherichia coli chaperone protein GroEL (Hsp60) and its regulator GroES are necessary for the proper folding of certain proteins , . [More information is available at EcoCyc: EG10599].