Recombinant Human Crystallin Alpha B | MyBioSource MBS142693 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Crystallin Alpha B

catalog :

MBS142693

quantity :

0.02 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142693

products type :

Recombinant Protein

products full name :

Recombinant Human Crystallin Alpha B

products short name :

Crystallin Alpha B

products name syn :

CRYAB Human; Crystallin Alpha B Human Recombinant; CRYA2; CTPP2; HSPB5; Crystallin Alpha B; CRYAB

other names :

alpha-crystallin B chain; Alpha-crystallin B chain; alpha-crystallin B chain; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; renal carcinoma antigen NY-REN-27; rosenthal fiber component; crystallin, alpha B; Alpha(B)-crystallin; Heat shock protein beta-5; HspB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component

products gene name :

CRYAB

other gene names :

CRYAB; CRYAB; MFM2; CRYA2; CTPP2; HSPB5; CMD1II; CTRCT16; HEL-S-101; CRYA2; HspB5

uniprot entry name :

CRYAB_HUMAN

host :

E Coli

sequence length :

175

sequence :

MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFPT STSLSPFYLR PPSFLRAPSWFDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHRKYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVT AAPKK.

purity :

Greater than 95.0% as determined by SDS-PAGE.

form :

The CRYAB protein (1mg/ml) solution contains 20mM Tris-HCl buffer pH-7.5, 50mM NaCl and 1mM EDTA. Sterile filtered colorless solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

products categories :

HEAT SHOCK PROTEINS; Recombinant Proteins; Crystallin

products description :

Description: Recombinant CRYAB produced in E Coli is a single,non-glycosylated polypeptide chain containing 175 amino acids and having a molecular mass of 20.1kDa. CRYAB is purified by proprietary chromatographic techniques. Introduction: Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of a-crystallins are an autokinase activity and participation in the intracellular architecture. Alpha-B is expressed widely in many tissues and organs and occurs in many neurological diseases.

ncbi gi num :

577019571

ncbi acc num :

NP_001276736.1

ncbi gb acc num :

NM_001289807.1

uniprot acc num :

P02511

ncbi mol weight :

20,159 Da

ncbi pathways :

Protein Processing In Endoplasmic Reticulum Pathway (167325); Protein Processing In Endoplasmic Reticulum Pathway (167190)

ncbi summary :

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

uniprot summary :

CRYAB: a major structural protein of the eye lens. A member of the small heat shock protein (sHSP also known as the HSP20) family. Alpha-B is expressed in the lens as well as other tissues. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Protein type: Chaperone; Heat shock protein. Chromosomal Location of Human Ortholog: 11q22.3-q23.1. Cellular Component: microtubule cytoskeleton; Golgi apparatus; cell surface; mitochondrion; cytoplasm; plasma membrane; actin filament bundle; cytosol; Z disc; nucleus. Molecular Function: identical protein binding; protein binding; protein homodimerization activity; metal ion binding; microtubule binding; unfolded protein binding; structural constituent of eye lens. Biological Process: lens development in camera-type eye; muscle development; protein folding; microtubule polymerization or depolymerization; stress-activated MAPK cascade; negative regulation of caspase activity; response to estradiol stimulus; tubulin folding; negative regulation of intracellular transport; muscle contraction; response to hydrogen peroxide; response to hypoxia; negative regulation of cell growth; protein homooligomerization; aging; negative regulation of apoptosis. Disease: Cataract 16, Multiple Types; Myopathy, Myofibrillar, Fatal Infantile Hypertonic, Alpha-b Crystallin-related; Myopathy, Myofibrillar, 2; Cardiomyopathy, Dilated, 1ii

size1 :

0.02 mg

price1 :

140 USD

size2 :

0.1 mg

price2 :

205

size3 :

1 mg

price3 :

1030

more info or order :

MyBioSource product webpage