protein

Recombinant Human Crystallin Alpha A

MBS142692

0.02 mg

140 USD

Recombinant Protein

Recombinant Human Crystallin Alpha A

Crystallin Alpha A

CRYAA Human; Crystallin Alpha A Human Recombinant; CRYA1; HSPB4; CRYAA; Crystallin Alpha A; Alpha-crystallin A chain; Heat shock protein beta-4

alpha-crystallin A chain; Alpha-crystallin A chain; alpha-crystallin A chain; crystallin, alpha-1; heat shock protein beta-4; human alphaA-crystallin (CRYA1); crystallin, alpha A; Heat shock protein beta-4; HspB4

CRYAA

CRYAA; CRYAA; CRYA1; HSPB4; CTRCT9; CRYA1; HSPB4; HspB4

CRYAA_HUMAN

E Coli

173

MDVTIQHPWF KRTLGPFYPS RLFDQFFGEG LFEYDLLPFL SSTISPYYRQ SLFRTVLDSGISEVRSDRDK FVIFLDVKHF SPEDLTVKVQ DDFVEIHGKH NERQDDHGYI SREFHRRYRLPSNVDQSALS CSLSADGMLT FCGPKIQTGL DATHAERAIP VSREEKPTSA PSS.

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

The CRYAA protein contains 20mM Tris-HCl buffer (pH 7.5), 50mM NaCl and 1mM EDTA. Sterile filtered colorless solution.

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

HEAT SHOCK PROTEINS; Recombinant Proteins; Crystallin

Description: Recombinant Human CRYAA produced in E Coli is a single, non-glycosylated polypeptide chain containing 173 amino acids and having a molecular mass of 19,909 Dalton. CRYAA is purified by proprietary chromatographic techniques. Introduction: Alpha crystallins are composed of two gene products ; alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein(sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional function of a-crystallins are an autokinase activity and participation in the intracellular architecture. The expression of alpha-A is preferentially restricted to the lens cell.

4503055

NP_000385.1

NM_000394.3

P02489

19,909 Da

Protein Processing In Endoplasmic Reticulum Pathway 167325!!Protein Processing In Endoplasmic Reticulum Pathway 167190

Mammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Defects in this gene cause autosomal dominant congenital cataract (ADCC). [provided by RefSeq, Jan 2014]

0.02 mg

140 USD