Recombinant Human Proguanylin | MyBioSource MBS142674 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Proguanylin

catalog :

MBS142674

quantity :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142674

products type :

Recombinant Protein

products full name :

Recombinant Human Proguanylin

products short name :

Proguanylin

products name syn :

Proguanylin Human; Proguanylin Human Recombinant; Guanylin Precursor; Guanylate cyclase activator 2A; Guanylate cyclase-activating protein 1; Gap-IGUCA2; STARA; GUANYLIN; Proguanylin

other names :

guanylin; Guanylin; guanylin; guanylate cyclase activator 2A (guanylin 2, intestinal, heat-stable); guanylate cyclase-activating protein 1; guanylate cyclase-activating protein I; prepro-guanylin; guanylate cyclase activator 2A (guanylin); Guanylate cyclase activator 2A; Guanylate cyclase-activating protein 1; Guanylate cyclase-activating protein I; GCAP-I

other gene names :

GUCA2A; GUCA2A; GUCA2; STARA; GCAP-I; GUCA2; GCAP-I

uniprot entry name :

GUC2A_HUMAN

host :

E Coli

sequence length :

115

specificity :

The proguanylin human recombinant is 100% homologous with the human proguanylin.

purity :

Greater than 95.0% as determined by SDS-PAGE. Three-step procedure using IMAC and size exclusion chromatography before and after refolding.

form :

Sterile filtered and lyophilized from 0.5 mg/ml in deionized H2O. Sterile Filtered White lyophilized (freeze-dried) powder.

storage stability :

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

other info2 :

Solubility: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.

products categories :

HORMONES; Hormones

products description :

Description: Proguanylin Human Recombinant produced in E Coli, is an 11 kDa protein containing 96 amino acid residues of the human proguanylin and 2 additional amino acid residues (underlined).The Proguanylin is purified by proprietary chromatographic techniques.GPVTVQDGNF SFSLESVKKL KDLQEPQEPR VGKLRNFAPI PGEPVVPILC SNPNFPEELK PLCKEPNAQE ILQRLEEIAE DPGTCEICAY AACTGC. Introduction: Heat-stable enterotoxins (STa) are small, cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a dramatic increase in the cGMP content of the cell; the increase, in turn, inhibits salt absorption and stimulates chloride secretion. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to the diarrhea and dehydration characteristic of enterotoxin activity. The identification of a receptor for STa on intestinal brush border membranes suggested the existence of an endogenous activator, described guanylin, a 15-amino acid peptide purified from rat small intestine, as a potential ligand for the STaR. This peptide shares sequence similarity with STa; see also uroguanylin. The molecular cloning of the human and mouse cDNAs encoding guanylin was reported. The sequences demonstrated that guanylin is present at the C-terminal end of a larger precursor protein. Expression in mammalian cells indicated that the 94- amino acid proguanylin is inactive. The biologically active guanylin can be released by either chemical or enzymatic treatment of proguanylin. By Northern blot analysis and in situ hybridization, showed that expression of guanylin mRNA is restricted to cells of the intestinal epithelium, specifically the Paneth cells at the base of the small intestinal crypts. These results demonstrate that guanylin is an endogenous activator of STaR isolated a cDNA encoding an apparent precursor of guanylin from a human intestinal cDNA library. The mRNA was expressed at high levels in human ileum and colon. In the mouse, interspecific backcross analysis used to map the Guca2 gene to the distal half of mouse chromosome 4 in a region of homology with human chromosome 1p. By fluorescence in situ hybridization mapped the GUCA2 gene to human 1p35-p34 Guanylin is thought to modulate intestinal water/electrolyte transport in a paracrine mode reported the nucleotide sequence of the gene, the characteristics of its circulating molecular form, and its localization in enterochromaffin cells of the gut. The gene, approximately 2.6 kb in size, consists of 3 exons interrupted by 2 introns. The hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3 kDa. Guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood.

ncbi gi num :

38176149

ncbi acc num :

NP_291031.2

ncbi gb acc num :

NM_033553.2

uniprot acc num :

Q02747

ncbi mol weight :

12,388 Da

ncbi pathways :

Myometrial Relaxation And Contraction Pathways 198759

size :

0.01 mg

price :

205 USD

more info or order :

MyBioSource product webpage