protein

Recombinant Human Prouroguanylin

MBS142639

0.01 mg

205 USD

Recombinant Protein

Recombinant Human Prouroguanylin

Prouroguanylin

GUCA2B Human; Prouroguanylin Human Recombinant; Guanylate cyclase activator 2B; UGN; GCAP-II; GUCA2B

guanylate cyclase activator 2B; Guanylate cyclase activator 2B; guanylate cyclase activator 2B; prepro-uroguanylin; guanylate cyclase activator 2B (uroguanylin); Guanylate cyclase C-activating peptide II; GCAP-II

GUCA2B

GUCA2B; GUCA2B; UGN; GCAP-II; GCAP-II; UGN

GUC2B_HUMAN

E Coli

112

The recombinant sequence is 100% homologous to the native human sequence.

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. Three-step procedure using IMAC and size exclusion chromatography before and after refolding.

Sterile filtered and lyophilized from 0.5 mg/ml in 50mM Phosphate buffer pH-7.4. Sterile Filtered White lyophilized (freeze-dried) powder.

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated thaw-freeze cycles and store at -80 degree C. Reconstituted protein can be stored at 4 degree C for a limited period of time.

Solubility: Add 0.2 ml of deionized H2O and let the lyophilized pellet dissolve completely.

HORMONES; Hormones

Description: Prouroguanylin Human Recombinant is 10 kDa protein containing 88 amino acid residues of the human prouroguanylin and 2 additional amino acid residues.GPVYIQYQGF RVQLESMKKL SDLEAQWAPS PRLQAQSLLP AVCHHPALPQ DLQPVCASQE ASSIFKTLRT IANDDCELCV NVACTGCLThe Prouroguanylin is purified by proprietary chromatographic techniques. Introduction: Prouroguanylin is a 112-amino-acid prohormone precursor of uroguanylin- mature biologically active 16-amino-acid peptide cleaved from its C-terminus. Guanylin binds to receptor-guanylate cyclases (CG-C) resulting in increased intracellular cGMP levels leading to CFTCR (Cystic Fibrosis Transmembrane Conductance Regulator) activation and subsequent rise in fluid and electrolyte secretion into the lumen.Prouroguanyline knock-out mice showed impaired renal excretion of external NaCl leading to elevated blood pressure independent of the level of diatary salt intake.Prouroguanylin is the predominant circulating molecular form found in circulation and in biological fluids.

6005774

NP_009033.1

NM_007102.2

Q16661

12,069 Da

Myometrial Relaxation And Contraction Pathways 198759

This gene encodes a member of the guanylin family, and is expressed in the stomach and intestine. This protein functions as an endogenous ligand for the guanylate cyclase-C receptor and stimulates an increase in cyclic GMP, a key component of several intracellular signal transduction pathways. It maybe involved in salt and water secretion into the intestinal lumen as well as the renal tubules, and thus regulate electrolyte homeostasis in these tissues. [provided by RefSeq, Oct 2011]

0.01 mg

205 USD