protein

Recombinant HIV-1 TAT Clade-B

MBS142615

0.002 mg

140 USD

Recombinant Protein

Recombinant HIV-1 TAT Clade-B

HIV-1 TAT Clade-B

HIV-1 TAT Clade-B; HIV-1 TAT Clade-B Recombinant; HIV-1 TAT Clade-B

E Coli

MEPVDPRLEP WKHPGSQPKT ACTNCYCKKC CFHCQVCFIT KALGISYGRK KRRQRRRPPQ GSQTHQVSLS KQPTSQSRGD PTGPKE.

Immunoreactive with all sera of HIV-1 infected individuals.

Greater than 90.0% as determined by HPLC analysis and SDS-PAGE.

Lyophilized with 0.1% glycerol. Sterile Filtered and lyophilized, though might appear as a solution as a result of the glycerol content.

Lyophilized HIV-1 TAT although stable at room temperature for 1 week, should be stored desiccated below -18 degree C. Upon reconstitution HIV-1 TAT should be stored at 4 degree C between 2-7 days and for future use below -18 degree C.For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Solubility: It is recommended to reconstitute the lyophilized HIV-1 TAT in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

VIRAL ANTIGENS- HUMAN IMMUNODEFICIENCY VIRUS; Viral Antigens; HIV

Description: HIV-1 TAT Recombinant- produced in E Coli is a single, non-glycosylated, polypeptide chain containing 86 amino acids encoded by two exons and having chain having a molecular mass of 14kDa. Introduction: Human immunodeficiency virus type-1 (HIV-1) regulatory Tat protein plays an essential role in viral replication (Jones KA, 1994) and infectivity (Arya SK, 1985; Fisher AG, 1986). In addition, during acute infection, Tat is released extracellularly by infected cells (Chang HC, 1997; Ensoli B, 1990) and is taken up by neighboring cells where it transactivates viral replication (Ensoli B, 1993) and increases virus infectivity. HIV-1 Tat activates transcription of HIV-1 viral genes by inducing phosphorylation of the C-terminal domain (CTD) of RNA polymerase II (RNAPII). Tat can also disturb cellular metabolism by inhibiting proliferation of antigen-specific T lymphocytes and by inducing cellular apoptosis. Tat-induced apoptosis of T-cells is attributed, in part, to the distortion of microtubules polymerization. LIS1 is a microtubule-associated protein that facilitates microtubule polymerization.

0.002 mg

140 USD

0.01 mg

205

0.1 mg

1030