catalog number :
MBS142543
products type :
Recombinant Protein
products full name :
Recombinant Hepatitis C Virus NS5 Genotype-1b
products short name :
Hepatitis C Virus NS5 Genotype-1b
products name syn :
HCV NS5 Genotype-1b; Hepatitis C Virus NS5 Genotype-1b Recombinant; HCV NS5 Genotype-1b
specificity :
Immunoreactive with sera of HCV-infected individuals.
purity :
HCV NS5 Genotype-1b protein is >95% pure as determined by SDS- PAGE (coomassie staining). HCV NS5 Genotype-1b protein was purified by proprietary chromatographic technique.
form :
1.5M urea, 25mM Tris-HCl pH 8.0, 0.2% Triton-X and 50% Glycerol. Sterile Filtered clear solution.
storage stability :
HCV NS5 Genotype-1b although stable at 4 degree C for 1 week, should be stored below -18 degree C. Please prevent freeze thaw cycles.
tested application :
ELISA (EIA), Western Blot (WB)
app notes :
HCV NS5 Genotype-1b antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
products categories :
VIRAL ANTIGENS- HEPATITIS C VIRUS; Viral Antigens; Hepatitis C
products description :
Description: The E Coli derived recombinant protein contains the HCV NS5 Genotype1b immunodominant regions, amino acids 2212-2313. The protein is fused to a GST tag at N-terminus. Introduction: HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by the HCV RNA polymerase, the HCV has an exceptionally high mutation rate, a factor that may help it elude the host's immune response. Hepatitis C virus is classified into six genotypes(1-6) with several subtypes within each genotype. The preponderance and distribution of HCV genotypes varies globally. Genotype is clinically important in determining potential response to interferon-based therapy and the required duration of such therapy. Genotypes 1 and 4 are less responsive to interferon-based treatment than are the other genotypes (2, 3, 5 and 6).