catalog number :
MBS142508
products type :
Recombinant Protein
products full name :
Recombinant Hepatitis B Surface Antigen preS2
products short name :
Hepatitis B Surface Antigen preS2
products name syn :
HBsAg preS2; Hepatitis B Surface Antigen, preS2 Recombinant; HBsAg preS2
other names :
HBsAg, partial; HBsAg; HBsAg
products gene name :
HBsAg
uniprot entry name :
Q09LT5_HBV
sequence :
ISSIFSRTGDPAPN.
MQWNSTTFHQALLDPKVRGLYFPAGGSSSGTVNPVPTTA
SP
purity :
HBsAg protein is >95% pure as determined by 10% PAGE (coomassie staining). HBsAg protein was purified by proprietary chromatographic technique.
form :
Lyophilized from 0.2um filtered (1mg/ml) solution in 20mM PB, pH7.4 and 50mM NaCl.
storage stability :
This lyophilized preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.
tested application :
ELISA (EIA)
app notes :
1. Immunochromatography (capture and conjugate). 2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS2. 3. ELISA.
other info2 :
Solubility: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at <-20 degree C. Further dilutions should be made in appropriate buffered solutions.
products categories :
VIRAL ANTIGENS- HEPATITIS B SURFACE ANTIGENS; Viral Antigens; HBsAg
products description :
Description: The Recombinant Hepatitis B Surface Antigen preS2 is a approximately 5.7 kDa, a single non-glycosylated polypeptide chain containing 55 amino acids. Purified by proprietary chromatographic technique. Introduction: Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, MHBs has a 55 amino acid region called preS2.
ncbi acc num :
ABI49990.1
ncbi mol weight :
6,135 Da