catalog number :
MBS142505
products type :
Recombinant Protein
products full name :
Recombinant Hepatitis B Surface Antigen preS1
products short name :
Hepatitis B Surface Antigen preS1
products name syn :
HBsAg preS1; Hepatitis B Surface Antigen, preS1 Recombinant; HBsAg preS1
other names :
HBsAg, partial; HBsAg; HBsAg
products gene name :
HBsAg
uniprot entry name :
Q09LT5_HBV
sequence :
MGGWSSKPRQGMGTNLSVPNPLGFFPDHQLDPAFGANSN
NPDWDFNPNKDHWPEAHQVGAGAFGPGFTPPHGGLLGWS
PQAQGILTTVPVAPPPASTNRQSGRQPTPISPPLRDSHP
QA.
purity :
HBsAg Protein is >95% pure as determined by 10% PAGE (coomassie staining). HBsAg protein was purified by proprietary chromatographic technique.
form :
HBsAg protein was lyophilized from 0.2um filtered (1mg/ml) solution in 20mM PB, pH 7.4, and 50mM NaCl.
storage stability :
This lyophilized HBsAg preparation is stable at 2-8 degree C, but should be kept at -20 degree C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 degree C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degree C to -70 degree C. Avoid repeated freeze/thaw cycles.
tested application :
ELISA (EIA)
app notes :
1. Immunochromatography (capture and conjugate). 2. Preparing monoclonal or polyclonal antibodies for HBsAg-preS1. 3. ELISA.
other info2 :
Solubility: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at
products categories :
VIRAL ANTIGENS- HEPATITIS B SURFACE ANTIGENS; Viral Antigens; HBsAg
products description :
Description: The E Coli derived Recombinant Hepatitis B Surface Antigen preS1 is a single non-glycosylated polypeptide chain containing 119 amino acids and having a molecular weight of 12.6 kDa. Introduction: Hepatitis B virus (HBV) is a human pathogen, causing serious liver disease. The HBV surface protein antigens (HBsAg) are comprised of three carboxyl co terminal HBs proteins termed large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs and MHBs also share the highly hydrophobic, repetitive, membrane spanning S domain. In addition, LHBs has a 119 amino acid region called preS1.
ncbi acc num :
ABI49990.1
ncbi mol weight :
6,135 Da