catalog number :
MBS142492
products type :
Native Protein
products full name :
Uracil DNA Glycosylase
products short name :
Uracil DNA Glycosylase
products name syn :
UNG; Uracil DNA Glycosilase; Uracil DNA Glycosilase; Uracil DNA Glycosylase; UNG
other names :
Uracil-DNA glycosylase; Uracil-DNA glycosylase
uniprot entry name :
Q6FHS8_HUMAN
host :
Escherichia Coli strain that carries the UNG gene from E Coli
form :
UNG solution in 10mM Tris-Hcl (pH-7.4 at 25 degree C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.1 mg/ml BSA and 50% glycerol. Sterile Filtered colorless solution.
storage stability :
Uracil DNA Glycosilase although stable at 15 degree C for 1 week, should be stored desiccated below -18 degree C. Please prevent freeze-thaw cycles.
other info1 :
10X UNG Reaction Buffer: 200mM Tris-HCl (pH 8.0 at 25 degree C), 10mM DTT and 10mM EDTA. Inhibition and Inactivation: Inactivated by heating at 95 degree C for 10min. Enzye activity is partially restored at temperatures lower than 55 degree C. Reaction Conditions: 1X UNG Reaction Buffer, incubate at 37 degree C.UNG is active over a broad pH rabge with an optimum at pH-8.0, doesn't require divalent cation, and is inhibited by high ionic strength (>200mM). The abasic sites formed in DNA by UNG may be cleaved by heat, alkali-treatment or endonucleases that cleave specifically at abasic sites. Unit Defenition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37 degree C. Specific Activity: The Specific Activity was found to be 5U/ ul. Unit Defenition: 1 Unit of the enzyme catalyzes the release of 1 nanomole of uracil-containing DNA template in 60 min at 37 degree C. Specific Activity: The Specific Activity was found to be 5U/ ul.
products categories :
ENZYMES; Enzymes; Glycosylase
products description :
Description: E Coli Uracil DNA Glycosilase (UNG) catalyses the release of free Uracil from Uracil-containing DNA. UNG efficiently hydrolyzes uracil from signle-stranded or double-stranded DNA, but not from oligomers (6 fewer bases).
ncbi mol weight :
33,926 Da