catalog number :
MBS142491
products type :
Recombinant Protein
products full name :
Recombinant Beta Lactamase
products short name :
Beta Lactamase
products name syn :
LACTB; Beta Lactamase Recombinant; b-Lactamase; EC 3.5.2.6; TEM precursor
other names :
beta-lactamase; Beta-lactamase
products gene name :
LACTB
other gene names :
Sbal_2086
uniprot entry name :
A3D4C4_SHEB5
sequence :
MHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW.
purity :
Greater than 90.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
Lyophilized from a concentrated (1 mg/ml) solution in water containing 20mM Phosphate buffer pH-7. Sterile Filtered White lyophilized (freeze-dried) powder.
storage stability :
Lyophilized Beta Lactamase although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution Beta Lactamase Recombinant should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
other info1 :
Unit Defenition: One unit will hydrolyze 1.0 umole of indicated substrate per min at pH 7.0 at 25 degree C. The International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units. Specific Activity: 1000IU/mg.
other info2 :
Solubility: It is recommended to reconstitute the lyophilized Beta Lactamase in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
products categories :
ENZYMES; Enzymes
products description :
Description: Beta-Lactamase TEM precursor Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 263 amino acids and having a molecular mass of 29 kDa. Beta Lactamase is purified by proprietary chromatographic techniques. Introduction: Beta-lactamase is a type of enzyme (EC 3.5.2.6) produced by some bacteria that is responsible for their resistance to beta-lactam antibiotics like penicillins, cephalosporins, cephamycins and carbapenems. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule''s antibacterial properties.
ncbi acc num :
YP_001050456.1
ncbi gb acc num :
NC_009052.1
ncbi mol weight :
43,037 Da
ncbi pathways :
Biosynthesis Of Secondary Metabolites Pathway (149204); Two-component System Pathway (47239); Two-component System Pathway (437); Beta-Lactam Resistance Pathway (1060599)