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company name :
MyBioSource
product type :
protein
product name :
Recombinant Human Ubiquitin Conjugating Enzyme E2M
catalog :
MBS142486
quantity :
0.02 mg
price :
205 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS142486
products type :
Recombinant Protein
products full name :
Recombinant Human Ubiquitin Conjugating Enzyme E2M
products short name :
Ubiquitin Conjugating Enzyme E2M
products name syn :
UBE2M Human; Ubiquitin Conjugating Enzyme E2M Human Recombinant; NEDD8-conjugating enzyme Ubc12; Ubiquitin-conjugating enzyme E2 M; NEDD8 protein ligase; NEDD8 carrier protein; UBC12; hUbc12; UBC-RS2
other names :
NEDD8-conjugating enzyme Ubc12; NEDD8-conjugating enzyme Ubc12; NEDD8-conjugating enzyme Ubc12; NEDD8 carrier protein; NEDD8 protein ligase; ubiquitin carrier protein M; ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast); ubiquitin-protein ligase M; yeast UBC12 homolog; ubiquitin-conjugating enzyme E2M; NEDD8 carrier protein; NEDD8 protein ligase; Ubiquitin-conjugating enzyme E2 M
products gene name :
UBE2M
other gene names :
UBE2M; UBE2M; UBC12; hUbc12; UBC-RS2; UBC12
uniprot entry name :
UBC12_HUMAN
host :
E Coli
sequence length :
183
purity :
Greater than 95% as determined by SDS-PAGE.
form :
The UBE2M solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 20% glycerol. Sterile filtered colorless solution.
products categories :
ENZYMES; Enzymes; Ubiquitin Conjugating Enzyme
products description :
Description: UBE2M Human Recombinant produced E Coli is a single polypeptide chain containing 207 amino acids (1-183) and having a molecular mass of 23.5kDa.UBE2M is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: UbcH12 is functional in in vitro NEDDylation reactions. It has been shown to form a thioester linkage with NEDD8 in the presence of the NEDD8 activating enzyme complex Uba3/APP-BP1. APP-BP1 binds to the amyloid precursor protein (APP) carboxy terminal domain and is important in conjunction with Uba3 and UbcH12 in driving cells through the S to M checkpoint. It was demonstrated to be the E2 responsible for the NEDDylation of the Cul-1 component of the SCF (?-TRCP) complex which is important as the E3-ligase in the ubiquitinylation of I?B?. NEDDylation of Cul-1 is essential for conjugation and processing of NF-?B p105 by SCF (?-TRCP) following phosphorylation of the complex. A dominant negative form of UbcH12, previously demonstrated to sequester NEDD8 and inhibit its conjugation, inhibits both conjugation and processing of p105, which is alleviated by wild-type UbcH12.
ncbi gi num :
4507791
ncbi acc num :
NP_003960.1
ncbi gb acc num :
NM_003969.3
uniprot acc num :
P61081
ncbi mol weight :
20,900 Da
ncbi pathways :
Adaptive Immune System Pathway 366160!!Antigen Processing: Ubiquitination Proteasome Degradation Pathway 366162!!Class I MHC Mediated Antigen Processing Presentation Pathway 366161!!Disease Pathway 530764!!Immune System Pathway 106386!!Loss Of Function Of SMAD2/3 In Cancer Pathway 906019!!Loss Of Function Of SMAD4 In Cancer Pathway 906017!!Loss Of Function Of TGFBR1 In Cancer Pathway 906025!!Loss Of Function Of TGFBR2 In Cancer Pathway 906022!!SMAD2/3 MH2 Domain Mutants In Cancer Pathway 906021
ncbi summary :
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
size :
0.02 mg
price :
205 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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