Recombinant Human Ubiquitin-Conjugating Enzyme E2K | MyBioSource MBS142480 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Ubiquitin-Conjugating Enzyme E2K

catalog :

MBS142480

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142480

products type :

Recombinant Protein

products full name :

Recombinant Human Ubiquitin-Conjugating Enzyme E2K

products short name :

Ubiquitin-Conjugating Enzyme E2K

products name syn :

UBE2K Human; Ubiquitin-Conjugating Enzyme E2K Human Recombinant; HIP2; Huntingtin Interacting protein 2; HYPG; Ubiquitin-conjugating enzyme E2-25K kDa; Ubiquitin-protein ligase; Ubiquitin carrier protein; LIG; HIP-2; E2(25K); DKFZp686J24237; OTTHUMP00000218440; EC 6.3.2.19

other names :

ubiquitin-conjugating enzyme E2 K isoform 2; Ubiquitin-conjugating enzyme E2 K; ubiquitin-conjugating enzyme E2 K; E2(25K); HIP-2; huntingtin interacting protein 2; huntingtin-interacting protein 2; ubiquitin carrier protein; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 KDA; ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-protein ligase; ubiquitin-conjugating enzyme E2K; Huntingtin-interacting protein 2; HIP-2; Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K; Ubiquitin-protein ligase

products gene name :

UBE2K

other gene names :

UBE2K; UBE2K; LIG; HIP2; HYPG; UBC1; E2-25K; HIP2; LIG; HIP-2; Ubiquitin-conjugating enzyme E2(25K); Ubiquitin-conjugating enzyme E2-25K

uniprot entry name :

UBE2K_HUMAN

host :

E Coli

sequence length :

157

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMANI AVQRIKREFK EVLKSEETSK NQIKVDLVDE NFTELRGEIA GPPDTPYEGG RYQLEIKIPE TYPFNPPKVR FITKIWHPNI SSVTGAICLD ILKDQWAAAM TLRTVLLSLQ ALLAAAEPDD PQDAVVANQY KQNPEMFKQT ARLWAHVYAG APVSSPEYTK KIENLCAMGF DRNAVIVALS SKSWDVETAT ELLLSN

purity :

Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

form :

The UBE2K protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH7.5) 1mM DTT, 50mM NaCl, and 10% glycerol. Sterile Filtered clear solution.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time.Please avoid freeze thaw cycles.

products categories :

ENZYMES; Enzymes; Ubiquitin Conjugating Enzyme

products description :

Description: UBE2K produced in E Coli is a single, non-glycosylated polypeptide chain containing 236 amino acids (1-200a.a.) and having a molecular mass of 26.5kDa.UBE2K is fused to a 36 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Introduction: UBE2K belongs to the ubiquitin-conjugating enzymes family which take part in many cellular processes such as selective protein degradation, DNA repair, cell cycle control, and sporulation. In this mechanism, the ATP-coupled activation and subsequent ligation of ubiquitin are catalyzed by separate enzymes functionally linked by ubiquitin carrier protein UBC1. In addition, UBE2K is involved in Alzheimer's disease, Huntington's disease and antigen processing through its interaction with amyloid-?, huntingtin, and MHC-heavy chain proteins.

ncbi gi num :

163660385

ncbi acc num :

NP_001104582.1

ncbi gb acc num :

NM_001111112.1

uniprot acc num :

P61086

ncbi mol weight :

17,447 Da

ncbi pathways :

Adaptive Immune System Pathway (366160); Antigen Processing: Ubiquitination Proteasome Degradation Pathway (366162); Class I MHC Mediated Antigen Processing Presentation Pathway (366161); Immune System Pathway (106386); Innate Immune System Pathway (106387); Negative Regulators Of RIG-I/MDA5 Signaling Pathway (187102); RIG-I/MDA5 Mediated Induction Of IFN-alpha/beta Pathways (187097); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

ncbi summary :

The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

UBE2K: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of Lys-48 -linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. In case of infection by cytomegaloviruses may be involved in the US11-dependent degradation of MHC class I heavy chains following their export from the ER to the cytosol. In case of viral infections may be involved in the HPV E7 protein- dependent degradation of RB1. Interacts with RNF138/NARF. Interacts with BRCA1. By aggregated low-density lipoprotein. Expressed in all tissues tested, including spleen, thymus, prostate, testis, ovary, small intestine, colon, peripheral blood leukocytes, T-lymphocytes, monocytes, granulocytes and bone marrow mononuclear cells. Highly expressed in brain, with highest levels found in cortex and striatum and at lower levels in cerebellum and brainstem. Belongs to the ubiquitin-conjugating enzyme family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Ubiquitin conjugating system; EC 6.3.2.19; Ubiquitin ligase; Ligase. Chromosomal Location of Human Ortholog: 4p14. Cellular Component: intermediate filament cytoskeleton; cytoplasm; nucleus; actin cytoskeleton. Molecular Function: protein binding; small conjugating protein ligase activity; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; ATP binding; ligase activity. Biological Process: ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; regulation of proteasomal ubiquitin-dependent protein catabolic process

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

5015

more info or order :

MyBioSource product webpage