catalog number :
MBS142479
products type :
Recombinant Protein
products full name :
Recombinant E Coli KBP-Type Peptidyl-Prolyl Cis-Trans Isomerase
products short name :
KBP-Type Peptidyl-Prolyl Cis-Trans Isomerase
products name syn :
SlyD E.Coli; FKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase E.Coli Recombinant; FKBP-Type Peptidyl-Prolyl Cis-Trans Isomerase; SlyD; SlyD E.Coli
other names :
FKBP-type peptidyl prolyl cis-trans isomerase (rotamase); FKBP-type peptidyl-prolyl cis-trans isomerase SlyD; FKBP-type peptidyl prolyl cis-trans isomerase (rotamase); Histidine-rich protein; Metallochaperone SlyD; Rotamase; Sensitivity to lysis protein D; WHP
other gene names :
slyD; slyD; ECK3336; JW3311; PPIase
uniprot entry name :
SLYD_ECOLI
sequence :
MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH.
purity :
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
form :
SlyD protein solution contains 20mM Tris pH-7.5. Sterile Filtered colorless solution.
storage stability :
SlyD although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
other info2 :
Biological Activity: Specific activity is > 220 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25C in Tris-Hcl pH8.0 using chymotrypsin.
products categories :
ENZYMES; Enzymes; Isomerase
products description :
Description: SlyD Recombinant produced in E Coli is a single, non-glycosylated polypeptide chain containing 196 amino acids and having a molecular mass of 21 kDa. Introduction: SlyD accessiton#: NP_755987 is a putative folding helper protein from the Escherichia coli cytosol, which has N-terminal prolyl isomerase domain of the FKBP type and a most likely unstructured C-terminal tail. SlyD is an important factor in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase.
ncbi acc num :
NP_417808.1
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
20,853 Da
ncbi summary :
HypB requires binding of SlyD for hydrogenase activity as SlyD helps facilitate nickel insertion. [More information is available at EcoGene: EG11663]. SlyD is a peptidyl prolyl cis/trans-isomerase (PPIase) and chaperone. [More information is available at EcoCyc: EG11663].