Recombinant Human Secreted Phospholipase A2-XII | MyBioSource MBS142471 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Secreted Phospholipase A2-XII

catalog :

MBS142471

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142471

products type :

Recombinant Protein

products full name :

Recombinant Human Secreted Phospholipase A2-XII

products short name :

Secreted Phospholipase A2-XII

products name syn :

PLA2G12 Human; Secreted Phospholipase A2-XII Human Recombinant; Group XIIA secretory phospholipase A2; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase GXII; GXII sPLA2; PLA2G12; sPLA2-XII; PLA2G12A

other names :

group XIIA secretory phospholipase A2; Group XIIA secretory phospholipase A2; group XIIA secretory phospholipase A2; GXII sPLA2; group XII secreted phospholipase A2; group XIIA secreted phospholipase A2; phosphatidylcholine 2-acylhydrolase 12A; phospholipase A2, group XII; sPLA2-XII; phospholipase A2, group XIIA; Phosphatidylcholine 2-acylhydrolase 12A

products gene name :

PLA2G12

other gene names :

PLA2G12A; PLA2G12A; GXII; ROSSY; PLA2G12; PLA2G12; GXII sPLA2; sPLA2-XII

uniprot entry name :

PG12A_HUMAN

host :

E Coli

sequence length :

189

purity :

Greater than 95% as determined by SDS PAGE.

form :

Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 0.01M Tris buffer pH 8.6. Filtered White lyophilized (freeze-dried) powder.

storage stability :

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

tested application :

Western Blot (WB)

other info2 :

Solubility: It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.

products categories :

ENZYMES; Enzymes; Secreted Phospholipase A2

products description :

Introduction: Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators.The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low molecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso- PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.

ncbi gi num :

21361944

ncbi acc num :

NP_110448.2

ncbi gb acc num :

NM_030821.4

uniprot acc num :

Q9BZM1

ncbi mol weight :

21,067 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194385)

ncbi summary :

Secreted phospholipase A2 (sPLA2) enzymes liberate arachidonic acid from phospholipids for production of eicosanoids and exert a variety of physiologic and pathologic effects. Group XII sPLA2s, such as PLA2G12A, have relatively low specific activity and are structurally and functionally distinct from other sPLA2s (Gelb et al., 2000 [PubMed 11031251]).[supplied by OMIM, Mar 2008]

uniprot summary :

PLA2G12A: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. Does not exhibit detectable activity toward sn-2-arachidonoyl- or linoleoyl- phosphatidylcholine or -phosphatidylethanolamine. Belongs to the phospholipase A2 family. Protein type: Phospholipase; Lipid Metabolism - linoleic acid; Lipid Metabolism - glycerophospholipid; Lipid Metabolism - ether lipid; Secreted, signal peptide; Endoplasmic reticulum; Lipid Metabolism - arachidonic acid; EC 3.1.1.4; Secreted; Lipid Metabolism - alpha-linolenic acid. Chromosomal Location of Human Ortholog: 4q25. Cellular Component: Golgi apparatus; endoplasmic reticulum; extracellular region. Molecular Function: calcium-dependent phospholipase A2 activity; calcium ion binding. Biological Process: phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; lipid catabolic process

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4140

more info or order :

MyBioSource product webpage