Recombinant Human Secreted Phospholipase A2-V | MyBioSource MBS142469 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Secreted Phospholipase A2-V

catalog :

MBS142469

quantity :

0.002 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142469

products type :

Recombinant Protein

products full name :

Recombinant Human Secreted Phospholipase A2-V

products short name :

Secreted Phospholipase A2-V

products name syn :

PLA2G5 Human; Secreted Phospholipase A2-V Human Recombinant; Calcium-dependent phospholipase A2; EC 3.1.1.4; Phosphatidylcholine 2-acylhydrolase; PLA2-10; Group V phospholipase A2; GV-PLA2; MGC46205; hVPLA(2); DKFZp686C2294; sPLA2-V; PLA2G5

other names :

calcium-dependent phospholipase A2; Calcium-dependent phospholipase A2; calcium-dependent phospholipase A2; Ca2+-dependent phospholipase A2; phosphatidylcholine 2-acylhydrolase 5; phospholipase A2, group V; Group V phospholipase A2; PLA2-10; Phosphatidylcholine 2-acylhydrolase 5

products gene name :

PLA2G5

other gene names :

PLA2G5; PLA2G5; FRFB; GV-PLA2; PLA2-10; hVPLA(2)

uniprot entry name :

PA2G5_HUMAN

host :

E Coli

sequence length :

138

purity :

Greater than 95% as determined by SDS PAGE.

form :

Filtered (0.4?m) and lyophilized in 0.5 mg/mL in 0.05M Acetate buffer pH4. Lyophilized (freeze-dried) powder.

storage stability :

Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.

other info2 :

Reconstitution: Add 0.1M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10ug/ml. In higher concentrations the solubility of this antigen is limited. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

products categories :

ENZYMES; Enzymes; Secreted Phospholipase A2

products description :

Description: Secreted Phospholipase A2-V Human Recombinant was produced with N-terminal His-Tag. PLA2G5 His-Tagged Fusion protein is 15.5 kDa containing 118 amino acid residues of the human secreted phospholipase A2-V and 16 additional amino acid residues His-Tag (underlined).MRGSHHHHHH GMASHMGLLD LKSMIEKVTG KNALTNYGFY GCYCGWGGRG TPKDGTDWCC WAHDHCYGRLEEKGCNIRTQ SYKYRFAWGV VTCEPGPFCH VNLCACDRKL VYCLKRNLRS YNPQYQYFPN ILCS. Introduction: Phospholipase A2 (PLA2) catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid (AA), a precursor of eicosanoids including prostaglandins and leukotrienes. The same reaction also produces lysophosholipids, which represent another class of lipid mediators.The secretory PLA2 (sPLA2) family, in which 10 isozymes have been identified, consists of low molecular weight, Ca2+-requiring secretory enzymes that have been implicated in a number of biological processes, such as modification of eicosanoid generation, inflammation, and host defense.This enzyme has been proposed to hydrolyze phosphatidylcholine (PC) in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.In mice, sPLA2 expression significantly influences HDL particle size and composition and demonstrate that an induction of sPLA2 is required for the decrease in plasma HDL cholesterol in response to inflammatory stimuli. Instillation of bacteria into the bronchi was associated with surfactant degradation and a decrease in large:small ratio of surfactant aggregates in rats.

ncbi gi num :

4505853

ncbi acc num :

NP_000920.1

ncbi gb acc num :

NM_000929.2

uniprot acc num :

P39877

ncbi mol weight :

15,674 Da

ncbi pathways :

Acyl Chain Remodelling Of PC Pathway (645321); Acyl Chain Remodelling Of PE Pathway (645317); Acyl Chain Remodelling Of PG Pathway (645328); Acyl Chain Remodelling Of PI Pathway (645326); Acyl Chain Remodelling Of PS Pathway (645324); Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365); Fat Digestion And Absorption Pathway (194385)

ncbi summary :

This gene is a member of the secretory phospholipase A2 family. It is located in a tightly-linked cluster of secretory phospholipase A2 genes on chromosome 1. The encoded enzyme catalyzes the hydrolysis of membrane phospholipids to generate lysophospholipids and free fatty acids including arachidonic acid. It preferentially hydrolyzes linoleoyl-containing phosphatidylcholine substrates. Secretion of this enzyme is thought to induce inflammatory responses in neighboring cells. Alternatively spliced transcript variants have been found, but their full-length nature has not been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

PLA2G5: PA2 catalyzes the calcium-dependent hydrolysis of the 2- acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes more efficiently L-alpha-1-palmitoyl-2-oleoyl phosphatidylcholine than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L- alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine, or L- alpha-1-stearoyl-2-arachidonyl phosphatidylinositol. May be involved in the production of lung surfactant, the remodeling or regulation of cardiac muscle. Defects in PLA2G5 are the cause of fleck retina, familial benign (FRFB). An autosomal recessive condition associated with a distinctive retinal appearance and no apparent visual or electrophysiologic deficits. Affected individuals are asymptomatic, but fundus examination reveals a striking pattern of diffuse, yellow-white, fleck-like lesions extending to the far periphery of the retina but sparing the foveal region. Belongs to the phospholipase A2 family. Protein type: Lipid Metabolism - arachidonic acid; Lipid Metabolism - glycerophospholipid; EC 3.1.1.4; Secreted, signal peptide; Secreted; Cell surface; Lipid Metabolism - linoleic acid; Lipid Metabolism - alpha-linolenic acid; Phospholipase; Lipid Metabolism - ether lipid. Chromosomal Location of Human Ortholog: 1p36-p34. Cellular Component: Golgi apparatus; cell surface; perinuclear region of cytoplasm; extracellular region; plasma membrane. Molecular Function: heparin binding; calcium-dependent phospholipase A2 activity; calcium ion binding. Biological Process: leukotriene biosynthetic process; platelet activating factor biosynthetic process; response to cAMP; response to cytokine stimulus; phospholipid metabolic process; glycerophospholipid biosynthetic process; phosphatidic acid biosynthetic process; arachidonic acid secretion; lipid catabolic process. Disease: Fleck Retina, Familial Benign

size1 :

0.002 mg

price1 :

140 USD

size2 :

0.01 mg

price2 :

205

size3 :

1 mg

price3 :

4140

more info or order :

MyBioSource product webpage