protein

Recombinant Human Deoxyribonuclease I

MBS142460

500 IU

140 USD

Recombinant Protein

Recombinant Human Deoxyribonuclease I

Deoxyribonuclease I

DNase Human; Deoxyribonuclease I Human Recombinant; EC 3.1.21.1; Deoxyribonuclease I; DNase I; DNL1; DRNI; FLJ38093; DNASE1

deoxyribonuclease-1; Deoxyribonuclease-1; deoxyribonuclease-1; DNase I, lysosomal; Dornase alfa; human urine deoxyribonuclease I; deoxyribonuclease I; Deoxyribonuclease I; DNase I; INN: Dornase alfa

DNase

DNASE1; DNASE1; DNL1; DRNI; DNL1; DRNI; DNase I

DNAS1_HUMAN

Chinese Hamster Ovarian Cells (CHO)

282

Each mg contains 150 ug calcium chloride dehydrate and 8.77 mg NaCl. Sterile liquid colorless solution at a concentration of 1mg/ml.

2 years when stored at 4 degree C, three weeks at 15 degree C, pH-6.3.

Unit Defenition: Dnase is generally assayed according to the photometric method developed by Kunitz. One Dnase unit results in an increase in absorbance at 260nm of 0.001/minute at 25 degree C when acting upon highly polymerized solution of DNA at pH-5. Also 0.005 Kunitz unit digests 1 ugof lambda phage DNA in 10 minutes at 37 degree C in50mM Tris, 1mMMg++, pH 7.8 in a 50ul reaction. Specific Activity: 1000IU/1mg.

ENZYMES; Enzymes; Nuclease

Description: Deoxyribonuclease I Human Recombinant produced in CHO is a glycosylated, polypeptide chain containing 260 amino acids and having a total molecular mass of 37,000 Dalton with a molecular formula of C1321H1995O396S9.DNase is purified by proprietary chromatographic techniques. Introduction: Deoxyribonuclease I Human Recombinant (rhDNase), an enzyme which selectively cleaves DNA. Recombinant Human Dnase is an endonuclease enzyme which splits phosphodiester linkages within polynucleotides, acting primarely on single stranded DNA (ssDNA), double stranded DNA (ddDNA) and chromatin. Dnase is activated by bivalent metals such as Mg+2 and Ca+2.Dnase enzymes are common reagents used in biochemical methods requiring diestion of DNA and recovery of RNA, or where DNA is to be removed without affecting structural proteins or enzymes. Dnase enzymes are also used in tissue culture to digest DNA from damaged cells, resulting in reduced viscosity, and for removal of membrane-bound DNA fragments.

21361254

NP_005214.2

NM_005223.3

P24855

19,771 Da

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

DNASE1: Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Defects in DNASE1 are a cause of susceptibility to systemic lupus erythematosus (SLE). A chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. Belongs to the DNase I family. Protein type: Secreted, signal peptide; Deoxyribonuclease; Secreted; EC 3.1.21.1; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16p13.3. Cellular Component: extracellular region; nuclear envelope; nucleus. Molecular Function: protein binding; deoxyribonuclease I activity; actin binding; endodeoxyribonuclease activity. Biological Process: apoptosis; DNA catabolic process, endonucleolytic. Disease: Systemic Lupus Erythematosus

500 IU

140 USD

1500 IU

205

10000 IU

645