Recombinant Human Cytochrome P450 2D6 | MyBioSource MBS142457 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Human Cytochrome P450 2D6

catalog :

MBS142457

quantity :

0.005 mg

price :

140 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS142457

products type :

Recombinant Protein

products full name :

Recombinant Human Cytochrome P450 2D6

products short name :

Cytochrome P450 2D6

products name syn :

CYP2D6; Cytochrome P450 2D6 Human Recombinant; Cytochrome P450 2D6; CYPIID6; P450-DB1; Debrisoquine 4-hydroxylase; CYP2D6; CPD6; CYP2D; CYP2D; CYP2DL1; P450C2D; MGC120389; MGC120390; LKM1; liver/kidney microsomal antigen 1

other names :

cytochrome P450 2D6 isoform 1; Cytochrome P450 2D6; cytochrome P450 2D6; cytochrome P450, family 2, subfamily D, polypeptide 7 pseudogene 2; cytochrome P450, family 2, subfamily D, polypeptide 8 pseudogene 2; cytochrome P450, subfamily II (debrisoquine, sparteine, etc., -metabolising), polypeptide 7 pseudogene 2; cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolising), polypeptide 8 pseudogene 2; cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing), polypeptide 6; cytochrome P450, subfamily IID (debrisoquine, sparteine, etc., -metabolizing)-like 1; cytochrome P450-DB1; debrisoquine 4-hydroxylase; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450, family 2, subfamily D, polypeptide 6; CYPIID6; Cytochrome P450-DB1; Debrisoquine 4-hydroxylase

products gene name :

CYP2D6

other gene names :

CYP2D6; CYP2D6; CPD6; CYP2D; CYP2DL1; CYPIID6; P450C2D; P450DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; P450-DB1; CYP2DL1

uniprot entry name :

CP2D6_HUMAN

host :

Sf9 Insect Cells

sequence length :

497

purity :

Greater than 40% as determined by SDS-PAGE.

form :

LKM-1 is supplied in 50mM Potassium phosphate buffer pH-7.5, 15% glycerol, 1mM DTT and 1% Triton X-100. Sterile filtered liquid formulation.

concentration :

0.35-0.6 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.

storage stability :

Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.

tested application :

Western Blot (WB)

app notes :

Western-Blot with monoclonal anti-LKM-1 antibody and autoimmune hepatitis patient sera.

other info2 :

Immunological Functions: 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels, including international reference serum NIBSC 66/387).

products categories :

ENZYMES; Recombinant Proteins; Cytochrome

products description :

Description: Cytochrome P450 2D6 Human Recombinant (also called liver/kidney microsomal antigen 1) produced in SF9, is a glycosylated, polypeptide chain having a molecular mass of 55,801 Dalton.The LKM1 is purified by proprietary chromatographic techniques. Introduction: Cytochrome P450 2D6 is a member of a complex family of microsomal enzymes (mono-oxygenases) present in the endoplasmic reticulum membrane, which perform detoxification reaction on xenobiotic compounds. Cytochrome P450 2D6 is the molecular target of autoantibodies against the "liver kidney microsomal antigen 1" (LKM 1) which has been classically defined by immunofluorescence microscopy. The presence of these autoantibodies is considered indicative of Autoimmune Hepatitis Type 2; LKM 1 antibodies have also been detected in patients with hepatitis C viral infection. The International Autoimmune Hepatitis Group therefore has subdivided the AIH type 2 into two subgroups: 2a with HCV and 2b without HCV. AIH 2a patients are often over 40 and predominantly male.The use of a purified recombinant Cytochrome P450 2D6 antigen allows the differentiation of autoimmune hepatitis from drug-induced hepatitis where transient autoantibodies to other P450 family members occur which cannot be differentiated by immunofluorescence techniques.

ncbi gi num :

392513721

ncbi acc num :

NP_000097.3

ncbi gb acc num :

NM_000106.5

uniprot acc num :

P10635

ncbi mol weight :

50,061 Da

ncbi pathways :

Biological Oxidations Pathway (105698); CYP2E1 Reactions Pathway (105705); Codeine And Morphine Metabolism Pathway (198831); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1127642); Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway (1127643); Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway (1127644); Defective CYP1B1 Causes Glaucoma Pathway (1127645)

ncbi summary :

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is known to metabolize as many as 25% of commonly prescribed drugs. Its substrates include antidepressants, antipsychotics, analgesics and antitussives, beta adrenergic blocking agents, antiarrythmics and antiemetics. The gene is highly polymorphic in the human population; certain alleles result in the poor metabolizer phenotype, characterized by a decreased ability to metabolize the enzyme's substrates. Some individuals with the poor metabolizer phenotype have no functional protein since they carry 2 null alleles whereas in other individuals the gene is absent. This gene can vary in copy number and individuals with the ultrarapid metabolizer phenotype can have 3 or more active copies of the gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

uniprot summary :

CYP2D6: Responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It is involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. Belongs to the cytochrome P450 family. Protein type: Xenobiotic Metabolism - drug metabolism - cytochrome P450; EC 1.14.14.1; Oxidoreductase. Chromosomal Location of Human Ortholog: 22q13.1. Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum. Molecular Function: iron ion binding; heme binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; arachidonic acid epoxygenase activity; drug binding; steroid hydroxylase activity; oxidoreductase activity; oxygen binding; monooxygenase activity. Biological Process: steroid metabolic process; coumarin metabolic process; monoterpenoid metabolic process; isoquinoline alkaloid metabolic process; negative regulation of binding; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; arachidonic acid metabolic process; alkaloid catabolic process; alkaloid metabolic process; heterocycle metabolic process; drug metabolic process. Disease: Drug Metabolism, Poor, Cyp2d6-related

size1 :

0.005 mg

price1 :

140 USD

size2 :

0.02 mg

price2 :

205

size3 :

1 mg

price3 :

3465

more info or order :

MyBioSource product webpage